时珍国医国药2026，Vol.37Issue(10)：1807-1811,5.DOI:10.70976/j.1008-0805.SZGYGY-2026-1002
半夏泻心汤调控p53/SAT1/ALOX15通路干预胃癌前病变的研究
Study on Banxia Xiexin Decoction(半夏泻心汤)in intervening gastric precancerous lesions via regulating the p53/SAT1/ALOX15 pathway
摘要
Abstract
Objective Expiloring the effect of Banxia Xiexin Decoction(半夏泻心汤,BXXXD)on regulating the p53/SAT1/ALOX15 pathway in rat gastic tissue and intervening in gastric precancerous lesions(GPL).Methods Twenty-four SD rats were randomly divided into a control group,a model group,and a BXXXD group.The control group received standard feeding,while the other two groups were modeled using the N-methyl-N'-nitro-N-nitrosoguanidine(MNNG)multi-factor composite method.After 19 weeks of continuous modeling,the model group and the BXXXD group continued to have free access to MNNG solution.Additionally,the BXXXD group received daily intragastric administration of BXXXD at a dose of 1 mL/100 g for 10 consecutive weeks.Gastric muco-sal histopathological changes were observed under light microscopy using HE staining.The mRNA expression levels of p53,SAT1,and ALOX15 were detected by RT-PCR.The protein expression levels of p53,SAT1,and ALOX15 were determined by Western blot(WB).Results HE staining revealed moderate to severe mucosal atrophy with some dysplasia in the model group,while the BXXXD group exhibited only mild atrophy and no dysplasia.Compared to the control group,the mRNA expression levels of p53,SAT1,and ALOX15 were significantly decreased in the model group(P<0.01).BXXXD intervention significantly increased the mRNA expres-sion levels of p53,SAT1,and ALOX15 compared to the model group(P<0.01).Similarly,the protein expression levels of p53,SAT1,and ALOX15 were significantly lower in the model group than in the control group(P<0.01),and these levels were higher after BXXXD intervention compared to the model group(P<0.01).Conclusion BXXXD may alleviate the pathological state of GPL in rats by regulating ferroptosis through the p53/SAT1/ALOX15 pathway.关键词
半夏泻心汤/胃癌前病变/铁死亡/p53/精脒/精胺N1-乙酰基转移酶1/花生四烯酸15-脂氧合酶Key words
Banxia Xiexin Decoction(半夏泻心汤)/Gastric precancerous lesions/Ferroptosis/p53/Spermidine/spermine N1-acetyltransferase 1/Arachidonate 15-lipoxygenase分类
医药卫生引用本文复制引用
马佳乐,王素盈,张馨元,高望,赵双梅,李慧臻..半夏泻心汤调控p53/SAT1/ALOX15通路干预胃癌前病变的研究[J].时珍国医国药,2026,37(10):1807-1811,5.基金项目
国家自然科学基金面上项目(82274442) （82274442）
天津市卫生健康委员会中医中西医结合科研课题(2023134) （2023134）
天津中医药大学第二附属医院育才计划(YC-FY202301) （YC-FY202301）
天津市教委科研计划项目(2024KJ043) （2024KJ043）
