时珍国医国药2026，Vol.37Issue(10)：1819-1829,11.DOI:10.70976/j.1008-0805.SZGYGY-2026-1004
基于代谢组学和肠道菌群探讨知柏地黄改良食疗方对幼龄性早熟大鼠的影响
Effects of Zhibai Dihuang Modified Dietary Formula(知柏地黄改良食疗方)on precocious puberty in juvenile rats:insights from metabolomics and gut microbiota
摘要
Abstract
Objective To investigate the therapeutic effects and underlying mechanisms of a Zhibai Dihuang Modified Dietary Formula(知柏地黄改良食疗方,ZDMDF)on precocious puberty in rats.Methods A precocious puberty model was established in Sprague-Dawley(SD)rats(n=8 per group)using danazol induction,with three experimental groups:control,model,and ZDMDF intervention.Serum levels of luteinizing hormone(LH),follicle-stimulating hormone(FSH),and estradiol(E2)were measured via ELISA.Uterine and ovarian organ indices were calculated,and histopathological changes were evaluated using paraffin-embedded hematoxylin-eosin(HE)staining.Gut microbiota(GM)composition and serum metabolite profiles were analyzed via 16S rRNA gene sequencing(Illu-mina MiSeq platform)and UHPLC-QTOF-MS-based non-targeted metabolomics,respectively.Results The model group exhibited sig-nificantly elevated serum LH,FSH,and E2 levels(P<0.01),along with increased uterine/ovarian indices(P<0.01),all of which were markedly attenuated by ZDMDF treatment(P<0.01).GM analysis revealed that ZDMDF restored the alpha-diversity(increased Shan-non and ACE indices,P<0.01),suppressed pro-inflammatory genera(e.g.,Escherichia-Shigella),and promoted the proliferation of probiotics(e.g.,Bifidobacterium,Akkermansia),with β-diversity analysis confirming microbiota realignment toward the control group.Metabolomic profiling revealed significant modulation of gonadotropin-releasing hormone(GnRH)signaling(P=0.040),arachi-donic acid metabolism-related pathways,and oxidative stress pathways,accompanied by restored short-chain fatty acids(e.g.,butyr-ate)and glutathione metabolic homeostasis.Procrustes analysis demonstrated strong concordance between GM and metabolomic shifts(P<0.01).Conclusion ZDMDF alleviates precocious puberty by remodeling GM structure,mitigating inflammation,and multi-target regulation of metabolic pathways.These findings provide mechanistic evidence supporting ZDMDF as a dietary intervention for preco-cious puberty and lay a foundation for its clinical translation.关键词
性早熟/知柏地黄改良食疗方/药食同源/肠道菌群/非靶向代谢组学Key words
Precocious puberty/Zhibai Dihuang Modified Dietary Formula(知柏地黄改良食疗方)/Medicinal and food homol-ogy/Gut microbiota/Non-targeted metabolomics分类
医药卫生引用本文复制引用
陈星,石强,刘英,何媛,敖梅英,刘潜..基于代谢组学和肠道菌群探讨知柏地黄改良食疗方对幼龄性早熟大鼠的影响[J].时珍国医国药,2026,37(10):1819-1829,11.基金项目
江西省科技厅省级科技专项资金项目(20243BCC31008) （20243BCC31008）
江西中医药大学科研培育项目(2023ZDPY001) （2023ZDPY001）
江西中医药大学校级重点领域科技创新团队项目(CXTD22014) （CXTD22014）
江西中医药大学校级教改项目(2022jzyx-2) （2022jzyx-2）
