时珍国医国药2026，Vol.37Issue(11)：2015-2021,7.DOI:10.70976/j.1008-0805.SZGYGY-2026-1103
基于JAK3/STAT1信号通路探讨解毒化瘀颗粒改善急性肝衰竭大鼠肝损伤的作用机制
Exploring the effect of Jiedu Huayu Granule(解毒化瘀颗粒)on liver injury in acute liver fail-ure rats via JAK3/STAT1 signaling pathway
摘要
Abstract
Objective To explore the interventional effect of Jiedu Huayu Granule(解毒化瘀颗粒,JDHYG)on liver injury in acute liver failure(ALF)rats and its effect on the JAK3/STAT1 signaling pathway.Methods 92 SPF-grade male SD rats were randomly divided into the normal group(20 rats),model group(36 rats),and JDHYG group(36 rats).The ALF rat model was established by using D-Galn/LPS,the normal group was given distilled water by gavage,and JDHYG group was given JDHYG solvent by gavage 72 hours before modeling.The survival rate of rats within 24 hours was observed.Serum and liver tissue samples were collected.ALT,AST,TBIL levels were detected by automatic biochemical analyzer,PT by a coagulometer,TNF-α and IL-6 by ELISA,the relative protein expression levels of JAK3 and STAT1 levels by Western blot,and the relative mRNA expression levels of JAK3 and STAT1 by qPCR.Liver tissue pathology was observed by optical microscope,and ultrastructural changes of mitochondria and endoplasmic reticulum by transmission electron microscope.Results Compared with the normal group,the liver cells in the model group showed necrosis,the structures of liver mitochondria and endoplasmic reticulum were damaged,and the survival rate of rats decreased(P<0.05).The levels of liver function,coagulation function,inflammatory response,the relative mRNA expression levels of JAK3 and STAT1,and the relative pro-tein expression levels were significantly increased(P<0.01),and liver mitochondria significantly decreased(P<0.01).Com-pared with the model group,the liver cells in the JDHYG group were repaired,the structures of liver mitochondria and endoplasmic reticulum were restored,the survival rate of rats increased(P<0.05).The levels of coagulation function,the rela-tive protein expression levels of JAK3 and STAT1 decreased(P<0.05).The levels of liver function,inflammatory levels,the relative mRNA expression levels of JAK3 and STAT1 significantly decreased(P<0.01),and liver mitochondria significantly increased(P<0.01).Conclusion JDHYG may inhibit inflammatory response,improve mitochondrial dysfunction,alleviate endoplasmic reticulum stress through the JAK3/STAT1 signaling pathway,thereby repairing the damaged liver in ALF rats.关键词
解毒化瘀颗粒/JAK3/STAT1信号通路/急性肝衰竭/炎症反应/线粒体/内质网Key words
Jiedu Huayu Granule(解毒化瘀颗粒)/JAK3/STAT1 signaling pathway/Acute liver failure/Inflammation/Mitochondria/Endoplasmic reticulum分类
医药卫生引用本文复制引用
潘李轶,陈月桥,陈玉,黄钰雯,裴浩,吴亚林,吴凤兰,叶绿萍,王娜..基于JAK3/STAT1信号通路探讨解毒化瘀颗粒改善急性肝衰竭大鼠肝损伤的作用机制[J].时珍国医国药,2026,37(11):2015-2021,7.基金项目
国家自然科学基金(82160888) （82160888）
广西自然科学基金(2022GXNSFAA035573) （2022GXNSFAA035573）
