时珍国医国药2026，Vol.37Issue(11)：2039-2044,6.DOI:10.70976/j.1008-0805.SZGYGY-2026-1106
乌梅丸改善肥胖小鼠血管内皮功能的作用机制研究
Study on the mechanism of Wumei Pill(乌梅丸)in improving vascular endothelial function of obese mice
摘要
Abstract
Objective To investigate the effects and mechanisms of Wumei Pill(乌梅丸,WMP)on endothelial function in obese mice and its impact on oxidized low-density lipoprotein(ox-LDL)-induced endothelial injury.Methods A high-fat diet(HFD)-induced obe-sity mouse model was established by feeding the mice for 24 weeks.Afterwards,the successfully established obese mice were randomly divided into the following groups:model group,the three WMP-treated groups at low,medium and high doses,and metformin group.The control group and model group were administered normal saline via gavage,while the other groups received the corresponding drug treatments via gavage for an additional 7 weeks.Body weight was recorded,using HE staining to observe morphological changes in the aorta,detecting serum lipid levels,and employing ELISA to measure inflammatory cytokine levels in each group.In vitro,HUVECs were cultured,and the proliferation,apoptosis,tubule formation,migration,and transwell migration abilities of the cells were assessed using CCK-8 assay,TUNEL assay,tubule formation assay,scratch assay,and transwell assay,respectively.Results Compared with the model group,the high-dose WMP group showed significant decrease in body weight,serum lipids,and malondialdehyde levels(P<0.05),while catalase activity was increased(P<0.01).Additionally,the levels of interleukin-1β and interleukin-6 were significantly reduced(P<0.001).HE staining revealed improved morphological changes in the aortic tissue.Compared to the ox-LDL group,the 10%WMP group showed enhanced cell proliferation and migration capacity(P<0.05)and a significant reduction in apoptosis(P<0.001).Conclusion WMP can improve endothelial function in obese mice and alleviate ox-LDL-induced endothelial cell injury,with its mechanism possibly involving reducing inflammation,antioxidant effects,promoting endothelial cell proliferation and migration,and inhibiting cell apoptosis.关键词
乌梅丸/肥胖/血管内皮细胞/炎症因子/细胞迁移/细胞凋亡Key words
Wumei Pill(乌梅丸)/Obesity/Endothelial cells/Inflammatory cytokines/Cell migration/Cell apoptosis分类
医药卫生引用本文复制引用
赵亮,呼延昕娜,张昊东,王晓翠..乌梅丸改善肥胖小鼠血管内皮功能的作用机制研究[J].时珍国医国药,2026,37(11):2039-2044,6.基金项目
宁夏医科大学中医学院一流学科建设项目(ZY0019110305) （ZY0019110305）
自治区全职引进高层次人才项目(2024BEH04075) （2024BEH04075）
宁夏医科大学校级科研项目(XZ2022007) （XZ2022007）
国家中医药管理局高水平中医药重点学科建设项目(zyyzdxk-2023209) （zyyzdxk-2023209）
