时珍国医国药2026，Vol.37Issue(11)：2045-2054,10.DOI:10.70976/j.1008-0805.SZGYGY-2026-1107
响应面法优化大黄和厚朴脂质体制备工艺及其结肠靶向性研究
Optimizing the preparation process of Radix et Rhizoma Rhei and Cortex Magnoliae Officina-lis liposomes by response surface methodology and study on their colon targeting
摘要
Abstract
Objective To optimize the formulation process of honokiol/rhein dual-loaded oral liposomes modified with chitosan(CS)and sodium alginate(SA)for colon targeting(HNK/RH-CS-SA Lip)and investigate their colon-targeting properties.Methods HNK/RH-CS-SA Lip were prepared using the thin-film dispersion method.With the phospholipid-to-cholesterol ratio,drug-to-lipid ratio,and ultrasonic power as evaluation indices,single-factor experiments and Box-Behnken response surface methodology were employed to screen the optimal preparation conditions.The morphological characteristics,particle size,and in vitro release behavior in different media of HNK/RH-CS-SA Lip were evaluated.The colon-targeting property in vivo was assessed through tissue distribution experiments in mice.Results The optimal preparation conditions for HNK/RH-CS-SA Lip were as follows:phospholipid-to-cholesterol mass ratio of 4.44:1,drug-to-phospholipid mass ratio of 1:9.77,ultrasonic power of 600 W,hydration medium of PBS,and hydration volume of 6 mL.Under the optimized conditions,the average encapsulation efficiency of HNK/RH-CS-SA Lip was 92.98%±0.74%,and the aver-age particle size was(340.78±2.01)nm.The liposomes exhibited sustained-release behavior and colon-targeted release characteris-tics.Conclusion The prepared HNK/RH-CS-SA Lip demonstrate good stability,uniform particle size distribution,high encapsulation efficiency,and colon-targeting properties.关键词
和厚朴酚/大黄酸/双载药脂质体/BoX-Behnken响应面/体外释放/结肠靶向Key words
Honokiol/Rhein/Dual-loaded liposomes/Box-Behnken response surface methodology/In vitro release/Colon targeting分类
医药卫生引用本文复制引用
谢燕秋,亢丽郦,梁梦莹,周杰,胡慧玲,王战国..响应面法优化大黄和厚朴脂质体制备工艺及其结肠靶向性研究[J].时珍国医国药,2026,37(11):2045-2054,10.基金项目
四川大学-达州校市合作专项资金项目(2022CDDZ-19) （2022CDDZ-19）
阿坝州科学技术局项目(R22YYJSYJ0022) （R22YYJSYJ0022）
四川省科技厅三区人才计划项目(2021-2023) （2021-2023）
