时珍国医国药2026，Vol.37Issue(11)：2055-2061,7.DOI:10.70976/j.1008-0805.SZGYGY-2026-1108
两种诱导子对党参不定根生长及多糖含量的影响
Effects of two inducers on the growth and polysaccharide content of adventitious roots of Codonopsis pilosula
摘要
Abstract
Objective To clarify the growth curve of the suspension culture of Codonopsis pilosula adventitious roots and to investigate the effects of two inducers,methyl jasmonate(MeJa)and chitosan(CTS),on the growth and polysaccharide content of the adventitious roots.Methods The dry and fresh weights of C.pilosula adventitious roots were measured at different days after inoculation.Two differ-ent types of inducers with varying concentrations were added to the adventitious root suspension culture system.Results The growth curve of C.pilosula adventitious roots showed an"S"shape.The optimal concentration of MeJa was 150 μmol/L with a suitable induc-tion time of 8 days;the optimal concentration of CTS was 50 mg/L with a suitable induction time of 4 days.Conclusions In the suspen-sion culture of C.pilosula adventitious roots,adding an appropriate concentration of MeJa or CTS on the 15th day of culture and induc-ing for a suitable duration can significantly increase the polysaccharide content of the adventitious roots.关键词
党参/不定根/诱导子/多糖/抗氧化酶Key words
Codonopsis pilosula/Adventitious roots/Inducers/Polysaccharides/Antioxidant enzymes分类
医药卫生引用本文复制引用
王琳佳,徐子杰,张延红,何春雨,郭清毅..两种诱导子对党参不定根生长及多糖含量的影响[J].时珍国医国药,2026,37(11):2055-2061,7.基金项目
国家自然科学基金(81960683) （81960683）
甘肃省高等学校产业支撑计划项目(2020C-09) （2020C-09）
