土木与环境工程学报（中英文）2026，Vol.48Issue(3)：1-12,12.DOI:10.11835/j.issn.2096-6717.2025.071
生物矿化用脲酶细菌的耐盐驯化
Salt tolerance domestication of urease bacteria for biocementation
摘要
Abstract
In engineering practice of microbial-induced carbonate precipitation(MICP),urease-producing bacte-ria(UPB)cannot tolerate high-salt concentrations of cementation solution(CS).Increasing the salt tolerance of UPB to CS can improve the efficiency and the application scope of MICP.In order to enhance the salt tolerance of Sporosarcina pasteurii,a gradual salt concentration gradient method was employed for its domestication.Growth characteristics in the domestication medium were evaluated through optical density tests,revealing a slowed growth rate in domesticated bacteria.Furthermore,salt tolerance tests,unconfined compressive strength(UCS)tests,and scanning electron microscopic analyses were conducted to investigate the calcium ion precipitation capacity,strength properties of bio-cemented sand columns,and microstructure of reinforced sam-ples among untamed bacteria(UB),domesticated bacteria(DB),and the control group.The results indicate the following:DB's tolerance to CS salt concentration exhibited an approximate increase of 0.25 mol/L.In the con-text of high concentration CS conditions,DB-treated samples demonstrated approximately double the UCS in comparison to UB,indicating a more efficient calcium ion precipitation process.Furthermore,DB resulted in a higher proportion of cauliflower-like calcium carbonate crystals under high concentration CS conditions.This crystal morphology is commonly associated with superior mechanical properties.These findings demonstrate that domestication effectively enhances bacterial biomineralization performance in high-salinity environments.关键词
微生物诱导碳酸盐沉淀/细菌驯化/细菌耐盐性/钙离子利用率/无侧限抗压强度/胶结液浓度Key words
microbial-induced carbonate precipitation(MICP)/bacteria domestication/salt tolerance of bacte-ria/calcium utilization rate/unconfined compressive strength(UCS)/cementation solution concentration分类
建筑与水利引用本文复制引用
肖子昊,孙雅娟,杨阳,刘汉龙..生物矿化用脲酶细菌的耐盐驯化[J].土木与环境工程学报（中英文）,2026,48(3):1-12,12.基金项目
National Natural Science Foundation of China(No.52108300) （No.52108300）
