西北农林科技大学学报（自然科学版）2026，Vol.54Issue(6)：42-52,11.DOI:10.13207/j.jnwafu.2026.06.005
花楸树(Sorbus pohuashanensis)与北京花楸(S.discolor)远缘杂交亲和性解析
Analysis of distant cross-compatibility between Sorbus pohuashanensis and S.discolor
摘要
Abstract
[Objective]The study aims to investigate the cross-compatibility between Sorbus pohuashanen-sis and S.discolor,to provide a theoretical basis for hybrid breeding of Sorbus.[Method]The mating system and reciprocal cross-compatibility between S.pohuashanensis and S.discolor were investigated through observ-ing flowering phenology,floral characteristics,artificial pollination experiments,and fluorescence microscope ob-servation of pollen tube growth.The authenticity of hybrid progenies was identified using 11 pairs of SSR prim-ers.[Result]The full-bloom stage and end of flowering period of S.pohuashanensis occurred later than those of S.discolor.with flowering periods overlapping in mid-April.The average ovule numbers were 6.70 for S.po-huashanensis and 9.27 for S.discolor,while average pollen grains per floret were 26 666.67 and 5 757.14,re-spectively.The pollen-ovule ratio was 3 980.10 and 621.05,respectively,and the outcrossing index was 4 for both species.The stigmas of both species showed high receptivity one day before flowering and on the first flow-ering day.Pollen of both species was able to adhere to and germinate on reciprocal stigmas.Crossing combina-tion of S.pohuashanensis×S.discolor exhibited higher compatibility than crossing combination of S.discolor×S.pohuashanensis.The artificial pollination experiment indicated that the mating systems of both species were outcrossing and self-incompatible,with average fruit-setting rates of 44.10%(S.pohuashanensis×S.discolor)and 49.04%(S.discolor×S.pohuashanensis).SSR marker analysis identified 114 hybrid progenies of S.po-huashanensis and S.discolor with 100%authenticity.Only three pairs of primer combinations(sorsb3,sorse10,sorsd39)were required to identify all the hybrid progenies.[Conclusion]Both S.pohuashanensis and S.discolor exhibited outcrossing and self-incompatible mating systems,and were reciprocally cross-compatible.关键词
花楸属/种间杂交/杂交种真实性/简单重复序列Key words
Sorbus/interspecific hybridization/hybrid authentication/SSR分类
农业科技引用本文复制引用
潘欣玥,杨文华,张川红,于雪丹,武宇霞,廖嘉,韩庆军,刘阳,栗宁宁,杜小娟,夏新合..花楸树(Sorbus pohuashanensis)与北京花楸(S.discolor)远缘杂交亲和性解析[J].西北农林科技大学学报（自然科学版）,2026,54(6):42-52,11.基金项目
国家自然科学基金面上项目(3207140400) （3207140400）
