现代妇产科进展2026，Vol.35Issue(5)：342-347,6.DOI:10.13283/j.cnki.xdfckjz.2026.05.032
血清miR-135a-5p、HOXA10与冻融胚胎移植患者子宫内膜容受性和妊娠结局的相关性研究
Study on the correlation between serum miR-135a-5p,HOXA10 levels with endometrial receptivity and pregnancy outcomes in patients undergoing frozen-thawed embryo trans-fer
摘要
Abstract
Objective:To explore the correlation between serum levels of microRNA-135a-5p(miR-135a-5p)and homeobox gene A10(HOXA10)with endometrial receptivity in-dicators and pregnancy outcomes in patients undergoing frozen-thawed embryo transfer(FET).Methods:From April 2022 to April 2024,130 patients who underwent FET in the Department of Reproductive Medicine of our hospital were enrolled as the study subjects.They were divided into pregnancy group and non pregnancy group based on the final pregnancy outcome.The ser-um levels of miR-135a-5p was detected by qRT-PCR.The serum levels of HOXA10 was detec-ted by ELISA.Endometrial receptivity indicators,including endometrial thickness(ET),endom-etrial volume(EV),resistant index(RI),and pulsatility index(PI)were measured by ultra-sound.Pearson and spearman correlation analysis were used to evaluate the correlation between indicators.Logistic regression analysis was performed to identify influencing factors.The predic-tive value of indicators for pregnancy outcomes was analyzed by receiver operating characteristic(ROC)curves,and the AUC values were compared using the DeLong test.Results:Compared with the pregnancy group,the non pregnancy group had higher serum miR-135a-5p level,ET,RI,and PI,but lower HOXA10 level and EV(P<0.05).In FET patients,serum miR-135a-5p was positively correlated with ET,RI,and PI,and negatively correlated with EV(P<0.05).Conversely,serum HOXA10 was negatively correlated with ET,RI,and PI,and positively corre-lated with EV(P<0.05).Elevated miR-135a-5p,elevated ET,decreased HOXA10,and de-creased EV were independent risk factors for non pregnancy in FET patients(P<0.05).The AUC values for predicting pregnancy outcome by miR-135a-5p,HOXA10,ET,and EV alone and their combination were 0.792,0.800,0.770,0.780,and 0.928,respectively.The AUC of the four-marker combination was significantly higher than that of any single marker(Z=2.915,2.795,3.326,3.060,P<0.05).Conclusion:Serum levels of miR-135a-5p and HOXA10 are closely associated with endometrial receptivity.Their combination with ET and EV can effective-ly predict pregnancy failure in FET patients.关键词
冻融胚胎移植/微小RNA-135a-5p/同源框基因A10/子宫内膜容受性/妊娠结局Key words
Frozen-thawed embryo transfer/MicroRNA-135a-5p/Homeobox gene A10/Endometrial receptivity/Pregnancy outcomes分类
医药卫生引用本文复制引用
万利静,程兰兰,张洁..血清miR-135a-5p、HOXA10与冻融胚胎移植患者子宫内膜容受性和妊娠结局的相关性研究[J].现代妇产科进展,2026,35(5):342-347,6.基金项目
河南省科技攻关项目(No:252102310221) （No:252102310221）
