现代中西医结合杂志2026，Vol.35Issue(7)：877-882,6.DOI:10.3969/j.issn.1008-8849.2026.07.001
蒙药清咽六味散对巨噬细胞的免疫调节作用研究
Immunomodulatory effect of Mongolian medicine Qingyanliuwei powder on macrophages
摘要
Abstract
Objective It is to investigate the effect of Mongolian medicine Qingyanliuwei powder on(QP)macrophage proliferation,migration and its immunomodulatory effects under inflammation status.Methods RAW 264.7 cells at loga-rithmic growth phase were taken and their proliferation rates after treatment with different concentrations of QP were as-sessed using the CCK-8 assay to screen out the optimal concentration for subsequent experiments.3 groups were established in the scratch experiment:a control group,a 25 μg/mL QP group,and a 50 μg/mL QP group,the scratch healing rates of cells in each group were calculated.4 groups were established in the lipopolysaccharide(LPS)stimulation experiment:the control group was treated with culture medium containing a small amount of PBS,the LPS group was treated with culture me-dium containing LPS at a final concentration of 100 ng/mL,the LPS+25 μg/mL QP group was treated with culture medium containing LPS at a final concentration of 100 ng/mL and QP at a concentration of 25 μg/mL,and the LPS+50 μg/mL QP group was treated with medium containing LPS at a final concentration of 100 ng/mL and QP at a concentration of 50 μg/mL.After 24 hours of culture,the mRNA expressions of macrophage polarization markers including inducible nitric oxide syn-thase(iNOS),arginase-1(Arg-1)and the inflammatory factors interleukin(IL)-1β and IL-6 in the cells were detected by qRT-PCR,the positive expression of iNOS in the cells was detected by immunofluorescence staining,and the levels of IL-1β and IL-6 in the cells were measured by ELISA.Results The cell proliferation rates in the 12.5 μg/mL,25 μg/mL,and 50 μg/mL QP groups were all significantly higher than that in the control group(all P<0.05);the cell scratch heal-ing rate in the 50 μg/mL QP group was significantly higher than that in the control group(P<0.05).Compared with the control group,the relative mRNA expressions of iNOS,IL-1β and IL-6,as well as the levels of IL-1β and IL-6 in the cells of the LPS group were significantly increased(all P<0.05),while the relative mRNA expression of Arg-1 was significantly reduced(P<0.05),and the fluorescence intensity of iNOS-positive expression in the cells was significantly enhanced.Compared with the LPS group,the relative mRNA expressions of iNOS and IL-1β in the cells of the LPS+25 μg/mL QP group were both significantly reduced(both P<0.05);the relative mRNA expressions of iNOS,IL-1β and IL-6,as well as the levels of IL-1β and IL-6 in the cells of the LPS+50 μg/mL QP group were significantly reduced(all P<0.05),while the relative mRNA expression of Arg-1 was significantly increased(P<0.05);the fluorescence intensities of iNOS-positive expressions were both significantly reduced in the LPS+25 μg/mL QP group and LPS+50 μg/mL QP group,and the relative mRNA expressions of iNOS and IL-1β in the cells of the LPS+50 μg/mL QP group were significantly lower than those in the cells of the LPS+25 μg/mL QP group(both P<0.05).Conclusion Qingyanliuwei powder can enhance macrophage proliferation and migration,and inhibit polarization towards the M1 phenotype and the secretion of pro-inflam-matory cytokines under inflammatory status.关键词
清咽六味散/巨噬细胞/炎症/免疫调节Key words
Qingyanliuwei powder/macrophages/inflammation/immunomodulation分类
医药卫生引用本文复制引用
付卓,赵晗,刘阳..蒙药清咽六味散对巨噬细胞的免疫调节作用研究[J].现代中西医结合杂志,2026,35(7):877-882,6.基金项目
内蒙古自治区一流学科科研专项项目(YLXKZX-NMD-008) （YLXKZX-NMD-008）
2023年度内蒙古自治区本级引进人才科研支持项目(RCQD202402) （RCQD202402）
内蒙古民族大学科研启动基金项目(KYQD23014) （KYQD23014）
