畜牧兽医学报2026，Vol.57Issue(5)：2625-2635,11.DOI:10.11843/j.issn.0366-6964.2026.05.020
母鸡产蛋后期贮精能力群体变异分析及高低贮精能力组代谢特征比较
Analysis of Population Variation of Hen's Sperm Storage Capacity in Late Laying Period and Comparison of Metabolic Characteristics between High and Low Sperm Storage Capacity Groups
摘要
Abstract
The purpose of this study was to analyze the population variation analysis of sperm storage capacity and compare the metabolic characteristics of high and low sperm storage capacity groups in the late laying period of White Leghorns laying hen.A total of 1 100 90-week-old White Leghorns laying hens were selected,and the phenotypes such as fertility rate and effective fertilization days were recorded.Based on the phenotype(egg laying rate＞75％),10 individuals with high fertility rate and 10 individuals with low fertility rate were selected,which were respectively defined as high and low sperm storage capacity groups.Serum was separated to detect the levels of estradiol(E2),progesterone(P4),prostaglandin F2α(PGF2α)and testosterone(T).At the same time,the differential metabolites between high and low sperm storage capacity groups were screened by serum non-targeted metabonomics,and KEGG pathway enrichment analysis was carried out.The results showed that the indexes of sperm storage capacity of hen population showed great individual variation in the late laying period,among which the coefficient of variation of intermittent fertilization intensity and the maximum number of consecutive fertile eggs were higher,which were 95.57％and 52.76％respectively.The levels of serum T and PGF2α were higher in the group with high sperm storage capacity(P＜0.05).A total of 58 differential metabolites were identified,of which 25 were up-regulated and 33 were down-regulated in the group with high sperm storage capacity.KEGG enrichment analysis showed that sperm storage capacity was highly correlated with lipid metabolism pathway.At the same time,the significant increase of serum diketone gulonic acid and 24-methylene cholesterol in the group with high sperm storage capacity is closely related to its antioxidant capacity.In conclusion,high sperm storage capacity group has stronger antioxidant capacity and lipid metabolism activity,thus preventing the damage of lipid peroxidation to sperm,and enhancing the sperm storage capacity.关键词
母鸡/贮精能力/群体变异/激素水平/代谢组学Key words
hen/sperm storage capacity/population variation/hormone level/metabolomics分类
农业科技引用本文复制引用
徐俊杰,邸科前,陈辉,石雷,李欣欣,李艳慧,范毅豪,吴星霖,王德贺,陈一凡,郝二英,赵晓钰..母鸡产蛋后期贮精能力群体变异分析及高低贮精能力组代谢特征比较[J].畜牧兽医学报,2026,57(5):2625-2635,11.基金项目
河北省现代农业产业技术体系建设项目(HBCT2024260201) （HBCT2024260201）
保定市"揭榜挂帅"项目(2024农101 （）
2024农206) （）
河北省省级科技计划(22326605D) （22326605D）
