畜牧兽医学报2026，Vol.57Issue(5)：2848-2857,10.DOI:10.11843/j.issn.0366-6964.2026.05.038
猪鼻支原体乳酸脱氢酶的表达及黏附功能研究
Expression and Adhesive Function of the Lactate Dehydrogenase of Mycoplasma hyorhinis
摘要
Abstract
Mycoplasma hyorhinis(Mhr)is a significant pathogen causing substantial economic losses to the pig industry.To investigate the role of lactate dehydrogenase(LDH)in the adhesion of Mhr to host cells,recombinant LDH(rLDH)was expressed,purified,and used to prepare hyperimmune rabbit serum.The surface expression of LDH on Mhr was confirmed by colony immunoblotting,flow cytometry,and Western blot.The cell-adhesion ability of rLDH was assessed using indirect immunofluorescence and microplate binding assays,and the inhibitory effect of anti-rLDH serum on Mhr binding to cell membrane proteins was evaluated.Finally,the binding capacity of rLDH to host plasminogen(Plg)and fibronectin(Fn)was examined.The results demonstrated that rLDH was successfully expressed and purified.Immunological assays confirmed that LDH is expressed on the Mhr surface.Functional assays revealed that rLDH adheres to PK-15 cells and binds to cell membrane proteins;notably,anti-rLDH antibodies significantly inhibited Mhr binding to cell membrane proteins.Far Western blot and microplate binding assays further demonstrated that rLDH binds to both Plg and Fn.These findings indicate that LDH is a surface-expressed protein of Mhr possessing multiple adhesive activities,playing a crucial role in colonization,invasion,and pathogenesis of Mhr.关键词
猪鼻支原体/乳酸脱氢酶/细胞黏附/纤溶酶原/纤连蛋白Key words
Mycoplasma hyorhinis/lactate dehydrogenase/cell adhesion/plasminogen/fibronectin分类
农业科技引用本文复制引用
卞程鹏,李卓,刘蓓蓓,于岩飞,陈蓉,韦艳娜,冯志新,王佳,熊祺琰..猪鼻支原体乳酸脱氢酶的表达及黏附功能研究[J].畜牧兽医学报,2026,57(5):2848-2857,10.基金项目
国家重点研发计划项目(2022YFD1800903) （2022YFD1800903）
国家自然科学基金项目(32470206) （32470206）
江苏省农业科技自主创新资金项目(CX(24)2001) （CX(24）
