畜牧兽医学报2026，Vol.57Issue(5)：2924-2935,12.DOI:10.11843/j.issn.0366-6964.2026.05.044
发酵黄芪调节鸡EVs上调干扰素和白细胞介素-2基因mRNA转录
Fermented Astragalus membranaceus Regulating Chicken EVs to Up-Regulate the mRNA Transcription of Interferon and Interleukin-2 Genes
摘要
Abstract
Astragalus polysaccharides have been shown to significantly modulate the expression levels of interferons and interleukin,yet the underlying mechanisms regulating these immune response pathways remain unclear.Recent studies indicate that extracellular vesicles(EVs)regulate multiple immune response pathways through the bioactive substances they carry.This study aims to investigate whether fermented Astragalus participates in the mRNA transcription of interferons and interleukin-2 genes by modulating EVs and their bioactive substances in chicken peripheral blood,thereby providing a theoretical foundation for understanding the antiviral effects of fermented Astragalus and developing novel antiviral drugs.Through the administration of fermented Astragalus,changes in the quantity of EVs in chicken peripheral blood were observed;the effects of EVs on the mRNA transcription of IFN-α,IFN-β,and IL-2 in chicken macrophages were assessed;differential miRNAs in EVs were analyzed to identify key regulatory miRNAs;and the consistency between target genes regulated by key miRNAs and the mRNA expression profile in chicken peripheral blood was further validated.The results demonstrated that the quantity of EVs in chicken peripheral blood significantly altered after the administration of fermented Astragalus,and these EVs markedly promoted the transcription of IFN-α,IFN-β,and IL-2 in chicken macrophages.Differential miRNA analysis revealed that miRNA-1388a is a crucial miRNA facilitating the transcription of IFN-α,IFN-β,and IL-2;further studies indicated that the target genes regulated by miRNA-1338a-3p were highly consistent with the mRNA expression profile in chicken peripheral blood.This study is the first to confirm that fermented Astragalus modulates the transcription of interferons and interleukin-2 genes through the regulation of chicken EVs and their mediated miRNAs.This discovery provides new insights into the antiviral mechanisms of fermented Astragalus and establishes a theoretical basis for the development of novel antiviral drugs.关键词
发酵黄芪/细胞外囊泡/干扰素/白介素/miRNAKey words
fermented Astragalus membranaceus/extracellular vesicles/interferon/interleukin/miRNA分类
农业科技引用本文复制引用
徐国伟,杨勤新,杨明星,王学智,韩松伟,陈富斌,孙研,李建喜,张景艳..发酵黄芪调节鸡EVs上调干扰素和白细胞介素-2基因mRNA转录[J].畜牧兽医学报,2026,57(5):2924-2935,12.基金项目
甘肃省重点人才项目(2025RCXM059) （2025RCXM059）
甘肃省兰州市科技重点项目(2023-1-4) （2023-1-4）
科技创新工程"中兽医与临床"25-LZIHPS-06 （）
兰州市人才创新创业项目(2022-RC-46) （2022-RC-46）
