畜牧与兽医2026，Vol.58Issue(6)：7-13,7.
槲皮素对绵羊M Ⅱ期卵母细胞玻璃化冷冻的影响
The effect of quercetin on vitrification of sheep M Ⅱ-stage oocytes
摘要
Abstract
This study aimed to investigate the antioxidant effects of quercetin(QUE)on sheep MII-stage oocytes during vitrification.After in vitro maturation for 24 h,sheep oocytes were randomly divided into five groups:the negative control group(fresh oocytes,without vitrifi-cation),the model group(vitrified,with 0 μmol/L QUE added to the vitrification solution),the low-dose group(vitrified,with 2.5 μmol/L QUE),the medium-dose group(vitrified,with 5 μmol/L QUE),and the high-dose group(vitrified,with 10 μmol/L QUE).After tha-wing,the oocytes from all groups were cultured in in vitro maturation medium supplemented with corresponding concentrations of QUE(0,2.5,5,and 10 μmol/L)for 0.5 h,and then the oocyte survival rate,reactive oxygen species(ROS)levels,glutathione(GSH)content,and mitochondrial membrane potential(JC-1)levels were assessed.The results showed that,compared with the negative control group,the survival rate,GSH content,and JC-1 levels were significantly decreased(P＜0.05),while the ROS levels were significantly increased(P＜0.05)in the model group,the low-dose group,and the high-dose group.However,no significant difference in the ROS levels was observed in the medium-dose group(P＞0.05).Among the vitrified groups,the low-dose and medium-dose groups significantly increased the oocyte survival rate,GSH content,and JC-1 levels(P＜0.05)and significantly decreased the ROS levels(P＜0.05),compared with the model group.In contrast,the high-dose group showed a significantly decreased survival rate,GSH content,and JC-1 levels(P＜0.05)and signif-icantly increased the ROS levels(P＜0.05),compared with the model group.In conclusion,the addition of a medium dose of QUE(5 μmol/L)during vitrification of sheep MII-stage oocytes significantly alleviated their oxidative stress,which might serve as a basis for impro-ving the efficiency of oocyte cryopreservation.关键词
槲皮素/卵母细胞/氧化应激/玻璃化冷冻/绵羊Key words
quercetin/oocyte/oxidative stress/vitrification/sheep分类
农业科技引用本文复制引用
华雨萱,刘伟俊,吕占民,王锐刚,武文,张云云,傅祥伟,王旭光,万鹏程,郭延华,王晶晶,唐玲,张振良,刘昱成,刘长彬,倪建宏,高磊..槲皮素对绵羊M Ⅱ期卵母细胞玻璃化冷冻的影响[J].畜牧与兽医,2026,58(6):7-13,7.基金项目
兵团重点领域科技攻关项目(2024AB017) （2024AB017）
兵团科技创新人才项目(2023CB007-07) （2023CB007-07）
新疆维吾尔自治区天山英才—青年科技拔尖人才项目(2022TSYCCX0124) （2022TSYCCX0124）
国家绒毛用羊产业技术体系项目(CARS-39-07) （CARS-39-07）
新疆维吾尔自治区羊产业技术体系项目(XJARS-09-26) （XJARS-09-26）
