烟草科技2026，Vol.59Issue(5)：17-26,43,11.DOI:10.16135/j.issn1002-0861.2025.0582
SFC-MS-MS法测定烟草中的游离态类脂成分
Determination of free lipoids in tobacco by SFC-MS-MS
摘要
Abstract
To simultaneously,accurately and rapidly determine free lipoid components in tobacco,an optimized supercritical fluid chromatography-tandem mass spectrometry(SFC-MS-MS)method was established to quantify free lipoid levels.Through systematic optimization of sample pretreatment conditions and instrumental parameters including chromatographic column type,mobile phase,modifier and ion source,accurate determinations of 15 lipoid components including α-cembra-trienediol,β-cembratrienediol,α-tocopherol,α-tocotrienol,β-sitosterol,campesterol,brassicasterol,cholesterol,stigmasterol,ergosterol,lanosterol,squalene,labd-13-ene-8,15-diol,cis-abienol and solanesol in tobacco were achieved.The results showed that:1)All analytes exhibited good linearity within the tested ranges,with all of the correlation coefficients(r)more than 0.99.2)Recoveries at different spiked levels ranged from 81.6%to 114.9%.Intra-and inter-day RSDs were 1.79%-6.29%and 1.74%-7.29%,respectively.Limits of detection and limits of quantification were 0.000 87-0.26 μg/mL and 0.002 9-0.85 μg/mL,respectively.3)Application of this method to various tobacco samples showed that this method could successfully detect the target lipoid components in different types of tobacco samples.Compared with traditional methods,this method exhibited strong matrix compatibility,high separation efficiency and sensitivity.It reduced organic solvent consumption and was eco-friendly.It could achieve simultaneous,accurate and rapid quantitative determination of main free lipid components in tobacco.关键词
烟草/类脂成分/游离态/超临界流体色谱-串联质谱/定量分析Key words
Tobacco/Lipoid component/Free state/Supercritical fluid chromatography-tandem mass spectrometry/Quantitative analysis分类
轻工纺织引用本文复制引用
于洁,王先颖,汪阳忠,戚大伟,陈敏,杨炘珂,吴达,顾文博..SFC-MS-MS法测定烟草中的游离态类脂成分[J].烟草科技,2026,59(5):17-26,43,11.基金项目
中国烟草总公司重大科技项目"国产雪茄烟叶品质风格的物质基础与配方维护模型构建研究"(110202201033[XJ-04]). （110202201033[XJ-04]）
