烟草科技2026,Vol.59Issue(5):56-65,10.DOI:10.16135/j.issn1002-0861.2025.0733
凉山州烟草主要病毒种类鉴定及病情分析
Identification and disease occurrence analysis of main tobacco viruses in Liangshan Prefecture
摘要
Abstract
To clarify the major types and occurrence of tobacco viruses in Liangshan Prefecture of Sichuan Province,diseased leaves of tobacco plants,leaves of weeds in tobacco fields and neighboring crops,aphids on tobacco,thrips on weeds and other crops were collected to analyze virus population structure and occurrence of major virus species by field survey,viral metagenomics analysis and PCR detection.The results showed that:1)Seven viruses belonging to four genuses were identified in diseased tobacco leaves.Among them,six were known tobacco viruses including cucumber mosaic virus(CMV),tobacco mosaic virus(TMV),chilli veinal mottle virus(ChiVMV),tobacco vein banding mosaic virus(TVBMV),potato virus Y(PVY),wild tomato mosaic virus(WTMV),and one,pepper vein yellows virus(PeVYV)belonging to Polerovirus,was novel.At the genus level,the abundance of Cucumovirus,Tobamovirus,Potyvirus and Polerovirus was 76.266 0%,1.251 1%,1.207 2%and 0.000 5%,respectively.Eleven plant viruses belonging to five genuses were identified in leaves of weeds in tobacco fields and neighboring crops.2)Six known tobacco viruses including TMV,PVY,CMV,tomato spotted wilt virus(TSWV),ChiVMV and tobacco leaf curl virus(TLCV)were identified by field survey.3)PCR detection and disease occurrence analysis showed that farming and wind damage caused TMV transmission between neighboring tobacco plants.关键词
烟草/病毒/宏病毒组/病情分析Key words
Tobacco/Virus/Viral metagenomics/Disease occurrence analysis分类
农业科技引用本文复制引用
江连强,殷乃勇,陈娟,申莉莉,焦裕冰,李莹,刘国,刘东阳,邓全,马鹏,高勇..凉山州烟草主要病毒种类鉴定及病情分析[J].烟草科技,2026,59(5):56-65,10.基金项目
四川省烟草公司凉山州公司科技项目"营养调节与免疫诱抗协同控制烟草病毒病技术开发与应用"(SCYC202311) (SCYC202311)
"凉山州2024年度新品种、新产品、新材料、新技术试验示范"(SCYC202415). (SCYC202415)