亚太传统医药2026，Vol.22Issue(6)：22-27,6.DOI:10.11954/ytctyy.202606004
杏黄止咳合剂通过PI3K/Akt/NF-κB信号通路治疗哮喘小鼠气道炎症机制研究
Effect and Mechanism of Xinghuang Zhike Mixture on Reducing Airway Inflammation in Asthmatic Mice Through PI3K/Akt/NF-κB Signaling Pathway
摘要
Abstract
Objective:To investigate the protective effect and mechanism of Xinghuang Zhike Mixture on airway inflam-mation in asthmatic mice.Methods:Mice were randomly divided into normal control group,model group,Xinghuang Zhike Mixture group and Xinghuang Zhike Mixture combined with budesonide group.Asthmatic mice model was estab-lished by ovalbumin sensitization and stimulation.In addition to the normal control group,the model group,Xinghuang Zhike Mixture group and Xinghuang Zhike Mixture combined with budesonide group were sensitized by intrabitoneal injection of ovalbumin and alumina hydrogen on the first,seventh and fourteenth day.From day twenty-one,mice were atomized and stimulated with ovalbumin solution continuously for fourteen days for 30 minutes each time.Xinghuang Zhike Mixture group was given intragastric administrationof Xinghuang Zhike Mixture(0.13mL/10g)one hour before stimulation,once a day for four weeks.Xinghuang Zhike Mixture combined with budesonide group was given intragas-tric administration of Xinghuang Zhike Mixture(0.13mL/10g)one hour before stimulation,and atomized inhalation of 0.01%budesonide 2mL for twenty minutes,once a day for four weeks.Normal control group and model group were given intragastric administration of 0.9%normal saline once a day for four weeks.The mice were anesthetized twenty four hours after last administration.The serum and alveolar lavage fluid of mice were collected for cell classification and counting.The pathological morphology of lung tissue was observed by hematoxylin-eosin staining.The contents of in-terleukin 5 and Interleukin-13 in alveolar lavage fluid and Interleukin-4 and Immunoglobulin E in serum were detected by Enzyme linked immunosorbent assay.Western Blot analysis protein expression levels in lung tissue of p-PI3K,PI3K,p-Akt,Akt,NF-κB、p56.Results:Xinghuang Zhike Mixture group and Xinghuang Zhike Mixture combined with budesonide group can significantly reduce(P<0.001)the number of white blood cells in the alveolar lavage fluid of asthmatic mice,pathological injury of lung tissue of asthmatic mice also improved.The levels of interleukin 5,interleu-kin-13,Interleukin-4 and Immunoglobulin Ereduced significantly(P<0.01).Xinghuang Zhike Mixture can significantly regulate(P<0.01)the expression of p-PI3K/PI3K,p-Akt/Akt,NF-κB p65 in asthmatic mice.Conclusion:Xinghuang Zhike Mixture may improve airway inflammation of asthmatic mice by inhibiting the expression of PI3K/Akt/NF-κB signaling pathway to protect lung tissue of mice.关键词
哮喘/气道炎症/小鼠/杏黄止咳合剂/磷脂酰肌醇3激酶/蛋白激酶B/核因子-κB信号通路Key words
asthma/airway inflammation/mice/Xinghuang Zhike mixture/PI3K/Akt/NF-κB signaling pathway分类
医药卫生引用本文复制引用
唐映红,王利敏,毛伟,费莹..杏黄止咳合剂通过PI3K/Akt/NF-κB信号通路治疗哮喘小鼠气道炎症机制研究[J].亚太传统医药,2026,22(6):22-27,6.基金项目
浙江省中医药科技计划项目(2023ZL680) （2023ZL680）
湖州市科技局公益性应用研究项目(2022GY36) （2022GY36）
