应用化学2026，Vol.43Issue(5)：719-728,10.DOI:10.19894/j.issn.1000-0518.250387
橙色荧光碳点的合成及其对过氧亚硝酸盐的荧光/比色双模式检测
Synthesis of Orange Fluorescent Carbon Dots for Fluorescence/Colorimetric Dual-Mode Detection of Peroxynitrite
摘要
Abstract
Peroxynitrite(ONOO-)plays an important role in cellular signal transduction and homeostasis;thereby,achieving the detection of ONOO-is crucial for early diagnosis and treatment of diseases.Herein,orange fluorescent carbon dots(O-CDs)were successfully synthesized by a one-step solvothermal method using rhodamine B and p-aminophenol as precursors.The O-CDs exhibited good selectivity and a sensitive response to ONOO-due to the oxidation of the amino/hydroxyl functional groups on their surface by ONOO-.Within the concentration ranges of 30～480 and 670～1070 nmol/L,the quenching efficiency of O-CDs showed a good linear correlation with the concentration of ONOO-,with the detection limit of 9 nmol/L.Meanwhile,the color of the solution gradually changed from pink red to dark blue.For the colorimetric mode,a good linear relationship between the absorbance change and ONOO-concentration was obtained in the range of 300～1000 nmol/L,with the detection limit of 90 nmol/L.Consequently,this work has achieved the fluorescent and colorimetric dual-mode specific recognition and detection of ONOO-.In addition,due to the excellent photostability and low toxicity of O-CDs,they have been successfully applied for the fluorescent imaging of ONOO-in PC-12 living cells,providing a valuable tool for elucidating the complex biological roles of ONOO-in related pathological processes.关键词
橙色荧光碳点/荧光和比色/过氧亚硝酸盐/细胞成像Key words
Orange fluorescent carbon dots/Fluorescent and colorimetric/Peroxynitrite/Cellular imaging分类
化学化工引用本文复制引用
郭建花,王松柏,邢晋羚,双少敏,董川..橙色荧光碳点的合成及其对过氧亚硝酸盐的荧光/比色双模式检测[J].应用化学,2026,43(5):719-728,10.基金项目
山西电子科技学院科研启动经费(No.2025KJ007)、山西省高等学校科技创新项目经费(No.2025L168)和国家自然科学基金(No.22274090)资助 Supported by the Startup Fund of Shanxi University of Electronic Science and Technology(No.2025KJ007),the Science and Technology Innovation Project Funding of Colleges and Universities in Shanxi Province(No.2025L168)and the National Natural Science Foundation of China(No.22274090) （No.2025KJ007）
