应用化学2026，Vol.43Issue(5)：755-761,7.DOI:10.19894/j.issn.1000-0518.250349
酪蛋白-载铁透明质酸复合物的结构及理化性质
Structure and Physicochemical Properties of Casein Iron Loaded Hyaluronic Acid Complex
摘要
Abstract
Iron deficiency anemia is an important nutritional deficiency disease worldwide.Developing efficient and safe iron fortified food is an effective strategy to improve iron nutrition.However,iron ions are prone to adverse interactions in food systems,resulting in low bioaccessibility.A composite delivery system based on casein(CAS)and iron loaded hyaluronic acid(HA-Fe)was constructed to improve the stability and bioavailability of iron ions.Compositions with m(CAS)∶m(HA-Fe)ratios of 10∶1 and 5∶1 were prepared.The structural properties of CAS were systematically characterized by nanoparticle size analyzer,zeta potential analysis,Fourier transform infrared spectroscopy(FT-IR),ultraviolet absorption spectroscopy and intrinsic fluorescence spectroscopy,and the release behavior and bioaccessibility of iron ions were evaluated by simulating gastrointestinal digestion model in vitro.Results showed that the stability of the system significantly improved after CAS-HA-Fe complexation,with increased absolute Zeta potential and more uniform particle size distribution.FT-IR revealed changes in secondary structure.Ultraviolet absorption spectroscopy and fluorescence spectra showed that the tertiary structure of protein changed and the tryptophan microenvironment tended to be hydrophobic.In vitro digestion experiments revealed that the bioavailability of iron ions reached its peak at 18.9%when the molar ratio of m(CAS)∶m(HA-Fe)was 5∶1.Casein iron loaded hyaluronic acid complex has good structural stability and iron protection ability,and can be used as a potential high-efficiency carrier for iron nutrition enhancement in functional foods.关键词
缺铁性贫血/酪蛋白/铁强化食品/透明质酸/生物可及性Key words
Iron deficiency anemia/Casein/Iron fortified food/Hyaluronic acid/Bioaccessibility分类
化学化工引用本文复制引用
刘鹏..酪蛋白-载铁透明质酸复合物的结构及理化性质[J].应用化学,2026,43(5):755-761,7.基金项目
吉林省教育厅"十三五"科学技术项目(No.JJKH20200486KJ)资助 Supported by the 13th Five Year Plan Science and Technology Project of Jilin Provincial Department of Education(No.JJKH20200486KJ) （No.JJKH20200486KJ）
