中草药2026，Vol.57Issue(11)：4147-4157,11.DOI:10.7501/j.issn.0253-2670.2026.11.007
基于超分子"印迹模板"理论分析五味消毒饮基准样品的质量属性
Analysis on quality attribute of substance benchmarks of Wuwei Xiaodu Yin based on supramolecular"imprinting template"theory
摘要
Abstract
Objective Based on the supramolecular"imprinting template"theory,this study investigates the autonomous interaction patterns of the component groups in Wuwei Xiaodu Yin(WXY,五味消毒饮)during the preparation process from crude herbs to reference samples and elucidates the quantitative transfer law of its quality attributes.Methods Fifteen batches of WXY reference samples were prepared via water decoction.A UPLC fingerprint analysis method was established to identify key chromatographic peaks and determine the contents of chlorogenic acid,esculetin,cichoric acid,neochlorogenic acid,cryptochlorogenic acid,isochlorogenic acid A,isochlorogenic acid B,isochlorogenic acid C,glehinnalin,and linarin were determined at 240 nm.Parameters such as transfer rate,extract yield,total statistical moment,and information entropy were calculated for both the herbs and the reference samples.Results The fingerprint similarity of the 15 batches of reference samples was greater than 0.9.Fourteen chromatographic peaks were identified,with 10 characteristic peaks defined.The extract yield ranged from 43.27％to 53.43％.The transfer rates of chlorogenic acid,esculetin,cichoric acid,neochlorogenic acid,cryptochlorogenic acid,isochlorogenic acid A,isochlorogenic acid B,isochlorogenic acid C,glehinnalin,and linarin were 34.25％—42.72％,51.52％—58.80％,28.21％—32.04％,56.26％—65.59％,40.63％—53.89％,22.35％—31.29％,38.39％—46.22％,34.02％—40.82％,18.64％—20.96％,and 28.55％—32.63％,respectively,with no discrete data observed.The relative standard deviations(RSDs)of the information entropy for the UPLC fingerprints of the five herbs and the reference samples were all less than 10％.The RSDs of mean chromatographic retention time of total quantum(MCRTT)and information entropy for WXY(crude herbs)were 3.90％and 2.25％,respectively.Conclusion Quality analysis of the reference samples based on the parameters of the supramolecular"imprinting template"provides a scientific basis for the quality control of WXY reference samples and the development of compound formulations.关键词
经典名方/五味消毒饮/基准样品/量值传递规律/超分子/印迹模板/质量属性/指纹图谱/绿原酸/秦皮乙素/菊苣酸/新绿原酸/隐绿原酸/异绿原酸A/异绿原酸B/异绿原酸C/格列风内酯/蒙花苷/总量统计矩/信息熵分类
医药卫生引用本文复制引用
肖强,肖美凤,袁琴,张子桉,陈凡,刘丹玲,黄端阳,曾慧,潘雪,贺福元..基于超分子"印迹模板"理论分析五味消毒饮基准样品的质量属性[J].中草药,2026,57(11):4147-4157,11.基金项目
国家自然科学基金资助项目(82274215) （82274215）
国家自然科学基金资助项目(82404815) （82404815）
湖南省自然科学基金资助项目(2025JJ80145) （2025JJ80145）
湖南省中医药管理局(B2024010) （B2024010）
湖南中医药大学研究生科研创新项目(2025CX083) （2025CX083）
湖南中医药大学研究生科研创新项目(2025XC096) （2025XC096）
湖南中医药大学研究生科研创新项目(CX20251199) （CX20251199）
