中草药2026，Vol.57Issue(11)：4238-4253,16.DOI:10.7501/j.issn.0253-2670.2026.11.014
荆防颗粒通过调控肺-肠轴及NOD-NF-κB信号通路改善哮喘模型小鼠气道炎症和气道重塑
Jingfang Granules alleviate airway inflammation and airway remodeling in asthmatic mice by regulating lung-gut axis and NOD-NF-κB signaling pathway
摘要
Abstract
Objective To investigate the intervention effect of Jingfang Granules(荆防颗粒)on airway inflammation and airway remodeling in asthmatic mice based on lung-gut axis,and elucidate the underlying molecular mechanism.Methods BALB/c mice were randomly divided into control group,model group,Jingfang Granules low-,medium-,high-dose(1,2,4 g/kg)groups and dexamethasone(1 mg/kg)group.A mouse asthma model was established by ip ovalbumin/aluminum hydroxide.After drug intervention,levels of interferon-γ(IFN-γ),tumor necrosis factor-α(TNF-α)in serum and interleukin-1β(IL-1 β),IL-6 in lung tissue were detected.Hematoxylin-eosin(HE),PAS and Masson staining were used to detect pathological changes in lung tissue of mice.Immunohistochemistry was used to detect mucin 5AC(MUC5AC)protein expression in lung tissue.Western blotting was used to detect the expressions of transforming growth factor-β1(TGF-β1)/Smad2/3 pathway,NOD nuclear factor-κB(NF-κB)signaling pathway in lung tissue and intestinal barrier related proteins.16S rRNA sequencing,untargeted metabolomics and proteomics analyses were integrated to systematically explore the potential mechanism of Jingfang Granules in asthma intervention from a multi-omics perspective.Results Compared with model group,Jingfang Granules significantly reduced the levels of TNF-α in serum and IL-1β,IL-6 in lung tissue of asthma model mice(P＜0.01),increased the level of IFN-γ in serum(P＜0.01),significantly reduced the infiltration of inflammatory cells in lung tissue,decreased the expression of MUC5AC in lung tissue,inhibited the activity of TGF-β1/Smad2/3 signaling pathway,thereby alleviating airway mucus secretion and airway remodeling.The results of multi-omics analysis further indicated that Jingfang Granules could upregulate the expressions of Occludin,Claudin1 and ZO-1,promote intestinal barrier repair,regulate gut microbiota imbalance and fecal metabolic disorders,and inhibit the abnormal activation of the NOD-NF-κB signaling pathway.Conclusion Jingfang Granules may ameliorate airway inflammation and airway remodeling in asthmatic mice by repairing intestinal barrier function,improving gut microbiota structure,correcting fecal metabolic disorders,and modulating NOD-NF-κB signaling pathway.关键词
荆防颗粒/哮喘/肠道菌群/肠-肺轴/代谢组学/NOD-NF-κB信号通路Key words
Jingfang Granules/asthma/gut microbiota/lung-gut axis/metabolomics/NOD-NF-κB signaling pathway分类
医药卫生引用本文复制引用
焦旭,姚景春,卢小艳,杨文龙,韩延忠,谭玉军,李市荣,姚方方,潘成龙,王颖,张贵民..荆防颗粒通过调控肺-肠轴及NOD-NF-κB信号通路改善哮喘模型小鼠气道炎症和气道重塑[J].中草药,2026,57(11):4238-4253,16.基金项目
山东省重点研发计划(重大科技创新工程)项目(2021CXGC010508) （重大科技创新工程）
