中风与神经疾病杂志2026，Vol.43Issue(5)：457-462,6.DOI:10.19845/j.cnki.zfysjjbzz.2026.0079
阿尔茨海默病病程与炎症因子相关性及多奈哌齐对重症阿尔茨海默病治疗作用的研究
Association between the course of Alzheimer disease and inflammatory factors and the therapeutic effect of done-pezil on severe Alzheimer disease
摘要
Abstract
Objective To investigate the expression levels of inflammatory factors and the mTOR signaling pathway in a mouse model of Alzheimer disease and their association with the course of the disease,as well as the therapeutic effect of donepezil in mice with severe Alzheimer disease.Methods C57BL6 mice,aged 8 weeks,were given intraperitoneal injection of different doses(60,120,and 180 mg/kg)of D-galactose to induce mild,moderate,and severe Alzheimer dis-ease,and the mice in the 180 mg/kg D-galactose group were given Aricept by gavage at a dose of 5 mg/kg as the treatment group.Behavioral experiments(water maze,Y maze,and novel object recognition test)were used to observe the change in cognitive ability;Western blotting was used to measure the expression levels of proteins associated with the mTOR sig-naling pathway;ELISA was used to measure the expression levels of the peripheral blood inflammatory factors interleukin-6(IL-6),interleukin-1β(IL-1β),and tumor necrosis factor-α(TNF-α);the Pearson test was used to investigate the cor-relation between inflammatory factors and the course of the disease in each group.Results The behavioral experiments showed that compared with the control group,the mice in each model group had different degrees of cognitive impairment(P<0.05),and donepezil treatment could reverse such changes(P<0.05).Western blotting showed varying degrees of abnormal activation of the mTOR signaling pathway in each model group(P<0.05),and the degree of activation increased with the increase in the dose of D-galactose(P>0.05);donepezil treatment could reverse such changes(P<0.05).ELISA showed that there were abnormal increases in the expression levels of the peripheral blood inflammatory factors IL-6,IL-1β,and TNF-α in each model group(P<0.05),and the increases in these factors increased with the dose of D-galactose;the expression levels of IL-6 and TNF-α were positively correlated with the dose of D-galactose.Conclusion The mTOR signaling pathway is abnormally activated in mice with Alzheimer disease,with abnormal in-creases in the levels of inflammatory factors,and the increases in inflammatory factors are associated with the course of the disease.Aricept has a therapeutic ef-fect in mice with severe Alzheimer disease.关键词
阿尔茨海默病/炎症因子/相关性/mTOR信号通路/多奈哌齐Key words
Alzheimer disease/Inflamma-tory factors/Correlation/The mTOR signaling pathway/Donepezil分类
医药卫生引用本文复制引用
刘福荣,唐超,舒敏,王佳良,沈男男..阿尔茨海默病病程与炎症因子相关性及多奈哌齐对重症阿尔茨海默病治疗作用的研究[J].中风与神经疾病杂志,2026,43(5):457-462,6.基金项目
国家自然科学基金(31801207) （31801207）
绍兴市卫生健康科技计划(2024SKY073) （2024SKY073）
浙江省药学会药品临床综合评价专项科研资助项目(2024ZYYL17) （2024ZYYL17）
