中国感染控制杂志2026，Vol.25Issue(5)：724-729,6.DOI:10.12138/j.issn.1671-9638.20263221
鲍曼不动杆菌生物膜形成、外膜蛋白OmpA/CarO表达与碳青霉烯类耐药相关研究
Correlation between biofilm formation,expression of outer membrane pro-tein OmpA/CarO and carbapenem resistance of Acinetobacter baumannii
摘要
Abstract
Objective To investigate the biofilm formation and expression of outer membrane proteins OmpA and CarO of Acinetobacter baumannii(A.baumannii),as well as their correlation with resistance to carbapenem anti-biotics.Methods The crystal violet staining method was used for qualitative and quantitative detection of biofilm forming ability of clinically isolated A.baumannii.The minimum inhibitory concentration(MIC)and minimum biofilm inhibitory concentration(MBIC)of imipenem against the strains were determined by the microdilution broth method.MBIC/MIC values were calculated,difference in drug resistance of bacterial strains in planktonic and biofilm conditions was compared.Total RNA was extracted from imipenem-resistant strains in two conditions,the relative expression levels of outer membrane protein OmpA and CarO genes were detected with fluorescence quanti-tative polymerase chain reaction(qPCR),and their potential impact on carbapenem resistance of A.baumannii was analyzed.Results Among the 108 A.baumannii strains,the detection rate of imipenem-resistant strains was 61.1%,detection rate of biofilm-positive strains was 93.5%,out of which 44(43.6%),45(44.5%)and 12(11.9%)strains showed weak,moderate and strong biofilm positivity,respectively.MBIC/MIC value analysis revealed that,regardless of whether the strains were resistant or sensitive,their resistance all increased after forming biofilm:for imipenem-resistant strains,most showed a 4-to 8-fold increase in resistance,while sensitive strains exhibited a 2-to 4-fold increase.Fluorescence qPCR showed that compared with the planktonic condition,the expression of outer membrane protein OmpA in A.baumannii increased significantly under biofilm condition(t=5.385,P<0.001),the expression of outer membrane porin protein CarO decreased significantly(t=-4.359,P<0.001).Conclusion The formation of biofilm can increase the carbapenem resistance of A.baumannii.The up-regulation of OmpA expression and down-regulation of CarO expression may be one of the key mechanisms of the increase of resistance.关键词
鲍曼不动杆菌/碳青霉烯类耐药性/生物膜/外膜蛋白/OmpA/外膜孔道蛋白/CarOKey words
Acinetobacter baumannii/carbapenem resistance/biofilm/out membrane protein/OmpA/outer membrane porin protein/CarO分类
医药卫生引用本文复制引用
董瑶,关丽娜,王朝莉,冯莉,谢勇恩..鲍曼不动杆菌生物膜形成、外膜蛋白OmpA/CarO表达与碳青霉烯类耐药相关研究[J].中国感染控制杂志,2026,25(5):724-729,6.基金项目
川北医学院国家级项目申报预研项目资助(CBY19-YZ02) （CBY19-YZ02）
