中国临床药理学杂志2026，Vol.42Issue(8)：1157-1163,7.DOI:10.13699/j.cnki.1001-6821.2026.08.017
基于铁自噬信号探究番茄红素对子宫内膜异位症大鼠炎症微环境影响的研究
Research of the effect of lycopene on the inflammatory microenvironment of endometriosis rats based on iron autophagy signal
摘要
Abstract
Objective To investigate the potential mechanism of lycopene mediating iron autophagy in improving the inflammatory response in endometriosis(EMs).Methods Fifty rats were randomly divided into sham group,model group(EMs model was constructed),experiment-L group(10.0 mg·kg-1 lycopene after modeled),experiment-H group(20.0 mg·kg-1 lycopene after modeled)and positive group(1.05 mg·kg-1 mifepristone after modeled),with 10 rats in each group.At the end of drug treatment,the volume of ectopic endometrial tissue were detected,and detected free iron content with a corresponding detection kit.The expressions of vascular endothelial growth factor A(VEGFA),interleukin(IL)-8,nuclear receptor coactivator 4(NCOA4)and microtubule-associated protein 1 light chain 3B(LC3B)were detected by immunofluorescence,or Western blot.Results The volumes of ectopic endometrial lesion tissues in the sham group,the model group,the experiment-L group,the experiment-H group and the positive group of rats were(0.00±0.00),(130.92±12.45),(97.55±10.56),(71.96±6.46),and(54.22±2.72)mm3,respectively;the levels of free iron were(262.89±23.51),(636.97±58.75),(523.63±46.13),(383.30±38.92)and(603.15±69.98)μmol·kg-1,respectively;the immunofluorescence intensities of VEGFA were(1 034.45±102.39),(3 628.16±104.65),(3 100.73±231.67),(2 193.07±293.16)and(3 418.68±377.98)a.u.,respectively;the relative expression levels of IL-8 protein were 0.22±0.05,0.79±0.10,0.62±0.10,0.49±0.06 and 0.71±0.07,respectively;the relative expression levels of NCOA4 protein were 0.33±0.04,1.12±0.13,0.85±0.10,0.61±0.09 and 1.05±0.08,respectively;the relative expression levels of LC3 Ⅱ/Ⅰ protein were 0.36±0.04,1.02±0.12,0.78±0.08,0.51±0.05 and 0.94±0.07,respectively.Compared the sham operation group with the model group,compared experiment-L and experiment-H groups with the model group,the differences of above indicators were all statistically significant(all P＜0.05).Conclusion Lycopene significantly inhibits inflammation and angiogenesis in EMs rats,thereby reducing the volume of ectopic lesions,which may be related to the inhibition of iron autophagy signaling.关键词
番茄红素/子宫内膜异位症/炎症反应/铁积累/自噬Key words
lycopene/endometriosis/inflammatory response/iron accumulation/autophagy分类
医药卫生引用本文复制引用
马会,孙丽佳,马月华,舒丽莎..基于铁自噬信号探究番茄红素对子宫内膜异位症大鼠炎症微环境影响的研究[J].中国临床药理学杂志,2026,42(8):1157-1163,7.基金项目
2024年政府资助临床医学优秀人才培养基金资助项目(ZF2024232) （ZF2024232）
