中国麻业科学2026，Vol.48Issue(2)：84-94,104,12.
一株拮抗钟器疫腐霉的类芽孢杆菌SEC-488的筛选、鉴定与全基因组分析
Identification and Whole-Genome Analysis of an Antagonistic Paenibacillus Strain SEC-488 against Phytopythium vexans
摘要
Abstract
Root brown rot caused by Phytopythium vexans is a major constraint on ramie yield and quality.To identify biocontrol microorganisms with antagonistic activity against P.vexans,we isolated an antagonistic bacterial strain,SEC-488,from ramie rhizosphere soil samples.Its antifungal activity was e-valuated using a dual-culture assay.Microscopic and scanning electron microscopy observations,biocon-trol-related functional assays,whole-genome sequencing,and bioinformatic analyses were conducted to assess its biocontrol potential.The results showed that SEC-488 significantly inhibited the mycelial growth of P.vexans in vitro,with an inhibition rate of(76.10±0.65)%.Microscopic observations revealed pronounced alterations in hyphal morphology and ultrastructure of the pathogen after exposure to SEC-488.Functional characterization indicated that SEC-488 exhibits protease and amylase activities and pos-sesses multiple biocontrol-related traits,including phosphate solubilization,potassium solubilization,ni-trogen fixation,and siderophore production.Based on morphological characteristics,physiological and bi-ochemical properties,16S rDNA gene phylogenetic analysis,and whole-genome average nucleotide iden-tity(ANI)and in silico DNA-DNA hybridization(isDDH)comparisons,SEC-488 was identified as a member of the genus Paenibacillus.Genome annotation revealed the presence of 310 carbohydrate-active enzyme-related genes and 17 biosynthetic gene clusters for secondary metabolite.These findings provide experimental evidence and a theoretical basis for the biocontrol of ramie root brown rot.关键词
苎麻/钟器疫腐霉/类芽孢杆菌/拮抗活性/全基因组分析/生物防治Key words
ramie/Phytopythium vexans/Paenibacillus/antagonistic activity/whole-genome anal-ysis/biological control分类
农业科技引用本文复制引用
邢濮厚,秦菁菁,冯佳,王芳,宋志强,刘华,颜健红,王吐虹,程毅..一株拮抗钟器疫腐霉的类芽孢杆菌SEC-488的筛选、鉴定与全基因组分析[J].中国麻业科学,2026,48(2):84-94,104,12.基金项目
长沙市自然科学基金(kq2502058) （kq2502058）
湖南省自然科学基金青年基金(2021JJ40648) （2021JJ40648）
国家自然科学基金青年基金(32502588) （32502588）
湖南省自然科学基金区域联合基金(2025JJ70599) （2025JJ70599）
中国农业科学院创新工程(CAAS-IBFC-2025-01) （CAAS-IBFC-2025-01）
