中国南方果树2026，Vol.55Issue(2)：102-107,120,7.DOI:10.13938/j.issn.1007-1431.20240551
基于荧光SSR标记的杧果杂交后代杂种真实性鉴定
Identification of mango hybrid progeny based on fluorescent SSR markers
摘要
Abstract
Hybrid breeding is the most important and effective method for developing new mango varieties,and the rapid and efficient identification of hybrid progeny is a prerequisite for the implementation of breeding programs.In this study,the hybrid progeny and their parents from three crosses including'Jinhuang'×'Renong No.1','Jinhuang'×'Irwin',and'Renong No.1'×'Tainong No.1'were used as experimental materials.With capillary electrophoresis,fluorescent polymorphic SSR markers were screened between the parents and further used to identify their hybrid progeny.The results showed that seven in-dividuals of the'Jinhuang'×'Renong No.1'cross at seven loci,six individuals of the'Jinhuang'×'Irwin'cross at six lo-ci,and eleven individuals of the'Renong No.1'×'Tainong No.1'cross at four loci amplified polymorphic bands from both parents,indicating all of them were hybrids.In the UPGMA clustering analysis,141132 and 141193 were clustered with'Jin-huang',1411100,130405,and 141166 were clustered with'Renong No.1',while 141182 and 141195 were clustered in a sep-arate category.All F1 individuals from both'Jinhuang'×'Irwin'and'Renong No.1'×'Tainong No.1'crosses were clustered with their respective paternal parents,showing a tendency of paternal inheritance.The results of this study provide important theoretical basis and reference for the identification of mango hybrids using fluorescent SSR molecular markers.关键词
杧果/杂种鉴定/SSR/毛细管电泳Key words
Mangifera indica L./hybrid identification/SSR/capillary electrophoresis引用本文复制引用
闫锦源,郑斌,王伟珊,王松标,马小卫,许文天,姚全胜,徐强,武红霞..基于荧光SSR标记的杧果杂交后代杂种真实性鉴定[J].中国南方果树,2026,55(2):102-107,120,7.基金项目
广东省种业振兴项目(2024-NPY-00-026) （2024-NPY-00-026）
中国热带农业科学院科技创新团队(CATASCXTD202405) （CATASCXTD202405）
云南省科技厅高原特色农业科技计划项目(202302AE090023) （202302AE090023）
农业农村部种质资源精准鉴定项目(19240684)资助. （19240684）
