中国农业科学2026，Vol.59Issue(10)：2061-2074,14.DOI:10.3864/j.issn.0578-1752.2026.10.001
小麦面粉色泽相关位点的育种利用效应分析
Analysis of the Breeding Utilization Effects of Loci Related to Wheat Flour Color
摘要
Abstract
[Objective]The flour color is a crucial index for evaluating wheat quality.Identifying superior allelic variations at loci associated with flour color and clarifying their breeding utilization effects will provide a foundation for molecular marker-assisted breeding of wheat flour color.[Method]Using functional markers,molecular detection was conducted on 12 flour color-related loci in 166 wheat varieties(lines),including the Psy-A1,Pds-B1,Lcye-A1,Lcye-B1,Lox-B1,Ppo-A1,Ppo-B1,Ppo-D1,Pod-A1,Pod-D1,Pod-2D genes,and the 1B/1R translocation.Combined with the phenotypic measurements of flour color parameters(Yellow pigment content(YPC),L* value,a* value,b* value and whiteness),the influence of different allelic variations on flour color were analyzed to comprehensively and systematically evaluate the breeding utilization effects of each locus.[Result]The flour color of the tested materials exhibited a wide variation range.The average value of YPC was 1.18 μg·g-1,with a range of 0.57-2.96 μg·g-1;The average value of L* was 90.29,ranging from 87.12 to 92.16;the average a* value was-0.86,varying between-1.78 and-0.09;the average b* value was 8.83,with a range of 5.21-14.69;and the average whiteness was 86.78,spanning from 81.35 to 90.30.Environment,genotype,and the interaction between genotype and environment all significantly influenced flour color,with genotype exerting the strongest effect on the phenotypic variations.Psy-A1 and 1B/1R translocation significantly affected YPC,L* value,a* value,b* value and whiteness;Lcye-B1 significantly influenced YPC,a* value,b* value and whiteness;Pds-B1 and Lox-B1 significantly impacted L* value,b* value and whiteness;Pod-2D significantly influenced L*value and whiteness;Lcye-A1 significantly affected L* value;Ppo-A1 and Ppo-D1 significantly influenced a* value.These nine loci had substantial impacts on flour color and exhibited great potential for breeding applications.Wheat varieties(lines)containing Psy-A1b,Pds-B1b,Lcye-A1b,Lcye-B1b,Lox-B1a,Ppo-A1b,Ppo-D1a,Pod-2D-GG,Pod-2D-AG and non-1B/1R translocation exhibited high brightness and whiteness flour color.These genotypes were designated as superior alleles,with distribution frequencies of 34.94%,20.48%,97.59%,66.27%,26.38%,50.91%,57.23%,48.80%,15.06%and 51.20%,respectively.As the number of superior alleles increased,the L* value,a* value and whiteness gradually increased,while YPC and b* value gradually decreased.The materials pyramiding 7-8 superior genes exhibited the optimal flour color.There were significant differences in flour color and the allelic variation frequencies of related genes among wheat varieties(lines)from different regions.A total of 22 varieties,such as Zhengyin1,Zimai12 and Wanmai19,carried more than seven superior alleles and could be used as parental materials for breeding wheat varieties with high brightness and whiteness flour.[Conclusion]Psy-A1,Pds-B1,Lcye-A1,Lcye-B1,Lox-B1,Ppo-A1,Ppo-D1,Pod-2D and 1B/1R translocation significantly influence flour color and exhibit strong breeding practicability.Twenty-two wheat varieties,such as Zhengyin1,Zimai12 and Wanmai19,can be used as excellent parents for flour color improvement.关键词
小麦/面粉色泽/分子标记/优异等位基因/育种利用Key words
Triticum aestivum/flour color/molecular marker/superior alleles/breeding utilization引用本文复制引用
翟胜男,韩冉,汪晓璐,李吉虎,刘建军,吕莹莹,何中虎,夏先春,马瑞峰,王颖,李法计,曹新有,李豪圣..小麦面粉色泽相关位点的育种利用效应分析[J].中国农业科学,2026,59(10):2061-2074,14.基金项目
山东省自然科学基金(ZR2023MC205和ZR2023MC155)、山东省泰山学者工程(tspd20221108)、山东省重点研发计划(2023CXPT079-2)、山东省农业科学院创新工程(CXGC2022E01)、国家小麦产业技术体系(CARS-03-06)、济南市"新高校20条"资助项目(202228067) （ZR2023MC205和ZR2023MC155）
