中国农业科学2026，Vol.59Issue(10)：2088-2108,21.DOI:10.3864/j.issn.0578-1752.2026.10.003
黍子NF-Y基因家族的鉴定及PmNF-YA8的功能分析
Identification of the NF-Y Gene Family and Functional Analysis of PmNF-YA8 in Broomcorn Millet
摘要
Abstract
[Objective]Nuclear factor Y(NF-Y)transcription factors regulate the expression of target genes by specifically binding to the CCAAT element in the promoter region of target genes,and play a role in plant responses to abiotic stresses such as drought and salinity.Broomcorn millet exhibits strong tolerance to drought and barrenness,serving as an indispensable crop for saline-alkali and marginal lands.Systematic elucidation of the functions of the NF-Y gene family in broomcorn millet will provide a theoretical basis for enriching the genetic theory of crop stress tolerance and promoting drought-resistant breeding practices.[Method]Genome data of broomcorn millet and bioinformatics approaches were employed to identify NF-Y family members,physicochemical properties,gene structures,construct phylogenetic trees,and predict conserved domains as well as cis-acting elements in promoters.Reverse Transcription Quantitative PCR(RT-qPCR)was used to determine the expression of these genes in different plant tissues,and the expression characteristics of PmNF-YA8 under drought stress were verified through transgenic functional validation in Arabidopsis thaliana.[Result]Based on the proso millet reference genome,33 PmNF-Y genes(including 11 PmNF-YA,12 PmNF-YB,and 10 PmNF-YC)were identified,encoding proteins with lengths of 122-571 amino acids,isoelectric points of 4.74-10.19,and molecular weights of 13.58-59.17 kDa.Chromosomal localization analysis showed that these 33 genes(PmNF-YA1-PmNF-YA11,PmNF-YB1-PmNF-YB12,and PmNF-YC1-PmNF-YC10)were distributed across 15 chromosomes.Two types of drought stress-responsive elements were predicted in the promoter regions of PmNF-Y family members.A total of 3,47,and 59 NF-Y orthologous gene pairs were identified between broomcorn millet and three other plant species(Arabidopsis thaliana,rice,and maize),respectively.RT-qPCR analysis revealed that PmNF-Y genes were widely expressed in tissues such as roots,stems,and leaves.The expression level of PmNF-YA8 was up-regulated in both XHS and NM9 varieties after drought stress.Transgenic Arabidopsis thaliana lines overexpressing PmNF-YA8 were generated,and phenotypic and physiological analyses showed that under 300 mmol·L-1 mannitol stress,the average root length of transgenic plants(14.03 mm)was longer than that of wild-type(WT)plants(9.07 mm).Additionally,transgenic plants had a lower MDA increase range(55.23%-57.12%)compared with WT plants(100.78%),with SOD and POD activities increased by 24.28%-27.43%and 163.57%-341.33%,respectively,and Pro content(151.31-175.14 μg·g-1)higher than that of WT plants(143.78 μg·g-1).[Conclusion]A total of 33 NF-Y family members were identified in broomcorn millet.The PmNF-YA8 gene was cloned from broomcorn millet and heterologously transformed into Arabidopsis thaliana.Its heterologous expression enhanced the drought stress tolerance of Arabidopsis thaliana plants by regulating the expression of stress-related genes,thus making PmNF-YA8 a key candidate gene for modulating drought tolerance in broomcorn millet.关键词
黍子/NF-Y/PmNF-YA8/表达模式/干旱胁迫Key words
broomcorn millet/NF-Y/PmNF-YA8/expression pattern/drought stress引用本文复制引用
宋裕祯,Bheel Chander Kumar,张颖星,王跃,Santra Dipak Kumar,曹晓宁,王瑞云..黍子NF-Y基因家族的鉴定及PmNF-YA8的功能分析[J].中国农业科学,2026,59(10):2088-2108,21.基金项目
现代农业产业技术体系建设专项(CARS-06-15.5-A16)、山西省现代农业产业技术体系建设(杂粮)专项资金(2026CYJSTX03-12)、山西省重点研发项目(2022ZDYF110) （CARS-06-15.5-A16）
