中国输血杂志2026，Vol.39Issue(5)：589-595,7.DOI:10.13303/j.cjbt.issn.1004-549x.2026.05.003
绿茶儿茶素在改善红细胞储存损伤中的作用
The role of green tea catechins in ameliorating erythrocyte lesion
摘要
Abstract
Objective To evaluate the effects of green tea extract(GTE)and its main catechin monomers on erythro-cyte lesion(such as hemolysis,decreased energy metabolism and oxidative stress)during in vitro storage,and to explore its potential as a novel additive for erythrocyte preservation solutions.Methods The composition of GTE was analyzed by high-performance liquid chromatography(HPLC).Using an in vitro simulated storage model,erythrocytes were stored in CPDA-1 preservation solution supplemented with GTE and the three most abundant catechin monomers(EGCG,ECG,EGC)for 60 days.Hemolysis rate and ATP content were dynamically monitored during storage.Flow cytometry was used to analyze phosphatidylserine(PS)exposure.Meanwhile,the protective effects of each component were verified in an acute oxidative stress model,and erythrocyte membrane stability was assessed by osmotic fragility test.Results After 60 days of storage at 4℃,the hemolysis rate at the end of storage in the GTE group was<0.8%,which was superior to that in the control group and the single catechin-supplemented groups.Erythrocyte osmotic fragility assay showed that GTE could en-hance the stability of erythrocyte membranes.In the acute oxidative stress experiment,the protective rate of GTE against e-rythrocyte injury exceeded 99%,which was better than that of the single catechin groups.At the initial stage of storage,ATP content decreased in all catechin-treated groups,but PS exposure was not significantly increased.Conclusion The addition of GTE can effectively alleviate storage lesions of erythrocytes,with efficacy superior to that of single catechins.GTE enhances the antioxidant capacity and membrane stability of stored erythrocytes.Our results provide new experimental evidence for the development of GTE-based erythrocyte preservation additives.关键词
红细胞/溶血/氧化应激/绿茶提取物/儿茶素/血液保存/抗氧化剂Key words
erythrocytes/hemolysis/oxidative stress/green tea extract/catechin/blood preservation/antioxidants分类
医药卫生引用本文复制引用
闫锡鹏,李玉佳,杨春晖..绿茶儿茶素在改善红细胞储存损伤中的作用[J].中国输血杂志,2026,39(5):589-595,7.基金项目
中国医学科学院医学与健康科技创新工程项目基金(2025-I2M-TS-12) （2025-I2M-TS-12）
中国输血协会威高基金(CSBT-WG-2022-01) （CSBT-WG-2022-01）
