中国畜牧兽医2026，Vol.53Issue(6)：2879-2888,10.DOI:10.16431/j.cnki.1671-7236.2026.06.009
牛活体采卵技术影响因素研究进展
Research Progress on Factors Affecting Bovine Ovum Pick-up Technology
摘要
Abstract
In dairy and beef cattle breeding,in vitro embryo production(IVP)has increasingly replaced in vivo-derived(IVD)embryos as the main source for bovine embryo transfer.As the initial and critical step of IVP,the efficiency of ovum pick-up(OPU)directly determines the number and quality of recovered oocytes,consequently,affects embryo yield.Therefore,a systematic analysis and optimization of environmental factors throughout the OPU process are essential for improving embryo production efficiency.This paper summarizes environmental factors influencing oocyte quantity,quality,and developmental competence across three stages:Pre-OPU,during OPU,and post-OPU.Pre-OPU factors include donor breed,nutritional status,hormonal treatment,age,and heat stress.Factors during OPU mainly involve the technical parameters of OPU equipment,aspiration frequency,etc.Post-OPU factors include cumulus cell coverage,cytoplasmic morphology,and the composition of the in vitro maturation medium.An integrated analysis of these factors enables systematic optimization of environmental management along the entire process—From donor body condition and hormonal regulation to OPU procedures and in vitro culture systems,providing a theoretical basis and practical guidance for standardizing and improving the efficiency of bovine embryo production technologies.关键词
牛/活体采卵/体外胚胎生产/环境因素/胚胎产量/发育潜能Key words
cattle/OPU/IVP/environmental factors/embryo yield/developmental potential分类
农业科技引用本文复制引用
钟海滨,张坤..牛活体采卵技术影响因素研究进展[J].中国畜牧兽医,2026,53(6):2879-2888,10.基金项目
国家重点研发计划项目(2023YFD1300501) （2023YFD1300501）
国家自然科学基金项目(32472902) （32472902）
