中国医科大学学报2026，Vol.55Issue(5)：437-442,6.DOI:10.12007/j.issn.0258-4646.2026.05.010
紫花牡荆素通过调节IL-10/STAT3信号通路减轻氧糖剥夺/复氧诱导的脑微血管内皮细胞损伤
Effect of casticin on oxygen-glucose deprivation/reoxygenation-induced brain microvascular endothelial cell injury by regulating IL-10/STAT3 signaling pathway
摘要
Abstract
Objective To explore the effect of casticin on oxygen-glucose deprivation/reoxygenation(OGD/R)-induced brain micro-vascular endothelial cell(BMEC)injury by regulating interleukin-10(IL-10)/signal transducer and activator of transcription 3(STAT3)signaling pathway.Methods Mouse BMECs were used to establish an OGD/R cell model and divided into OGD/R,low-concentration casticin,medium-concentration casticin,high-concentration casticin,and high-concentration casticin+AS101 groups.BMECs without OGD/R induction were used as the control group.Cell proliferation was detected using the MTT assay,cell apoptosis using flow cytometry,angiogenesis using tube formation assay,transendothelial electrical resistance(TEER)using Transwell chambers,endothelial permeability using FITC-dextran assay,and the expression of IL-10,p-STAT3,and STAT3 proteins in BMECs using Western blotting.Results Com-pared with the control group,BMEC viability,tubular structure formation,TEER,and expression of IL-10 and p-STAT3 proteins were decreased in the OGD/R group,and the apoptosis rate and endothelial permeability were increased(P<0.05).Compared with the OGD/R group,BMEC viability,tubular structure formation,TEER,and expression of IL-10 and p-STAT3 proteins were increased in the low-,medium-,and high-concentration casticin groups,whereas the apoptosis rate and endothelial permeability were decreased(P<0.05).Compared with the high-concentration casticin group,BMEC viability,tubular structure formation,TEER,and expression of IL-10 and p-STAT3 proteins were decreased in the high-concentration casticin+AS101 group,and the apoptosis rate and endothelial permeability were increased(P<0.05).Conclusion Casticin may alleviate OGD/R-induced injury in BMECs by regulating IL-10/STAT3 signaling pathway.关键词
紫花牡荆素/白细胞介素-10/信号转导及转录激活因子3/氧糖剥夺/复氧/脑微血管内皮细胞Key words
casticin/interleukin-10/signal transducer and activator of transcription 3/oxygen-glucose deprivation/reoxygenation/brain microvascular endothelial cell分类
医药卫生引用本文复制引用
唐永军,张煜华,玉山江·朱玛合,迪力木拉提·依斯热依力,西合热扎提·阿布力米提..紫花牡荆素通过调节IL-10/STAT3信号通路减轻氧糖剥夺/复氧诱导的脑微血管内皮细胞损伤[J].中国医科大学学报,2026,55(5):437-442,6.基金项目
自治区重点实验室新疆神经系统疾病研究重点实验室开放课题(XJDX1711-2405) （XJDX1711-2405）
