中国药理学通报2026，Vol.42Issue(5)：974-983,10.DOI:10.12360/CPB202512149
加味当归芍药散对肺炎链球菌诱导幼鼠肾损伤的保护作用
Protective effect of Jiawei Danggui Shaoyao San against Streptococcus pneumoniae-induced renal injury in young rats
摘要
Abstract
Aim To explore the renal protective ef-fects of Danggui Shaoyao San(DSS)and its modified prescription(Herba Ephedrae and Radix Astragali)on an infection-related glomerulonephritis(IRGN)ani-mal model via establishing repeated nebulization of Streptococcus pneumoniae(S.pn)in immature rats.Methods SPF male SD rats at 3-week-old were ran-domly divided into two groups,and the IRGN model was established by two ways,namely,aerosol injec-tion and swimming injection,and then the rats were given intragastric administration of DSS,Jiawei DSS,Ephedra and Astragalus,and oseltamivir.Urinary pro-tein/creatinine ratio,serum biochemical indicators and inflammatory factors in bronchoalveolar lavage fluid were dynamically detected.HE staining and transmis-sion electron microscopy were used to observe the pa-thology of lung and kidney and the ultrastructure of renal tissue.MAC and IgM deposition were detected by immunohistochemistry.Results The weight of rats decreased,the pathological injury of lung and kidney,the increase of inflammatory factors,the abnormality of urinary protein/creatinine ratio and serum biochemi-cal indexes,the increase of IgM deposition,the de-crease of Podocin and the increase of neuraminidase were observed in rats of the bacteria group and the swimming combined bacteria group.The injury of the swimming combined bacteria group was more obvious,suggesting that the model was successfully constructed and the swimming combined bacteria group was se-lected;DSS group,Jiawei DSS group and Ephedra and Astragalus group could improve renal injury,regu-late urinary protein/creatinine ratio,serum biochemis-try and other related indicators,among which Jiawei DSS had the best effect.Conclusions The compound model of IRGN is successfully established,which con-firms that DSS and its modified prescriptions have pro-tective effects on IRGN induced by S.pn in young rats,and it provides experimental evidence for the study of related pathological mechanisms and the pre-vention and treatment of traditional Chinese medicine.关键词
肺炎链球菌/肺炎/肾脏损伤/神经氨酸酶/动物模型/当归芍药散Key words
Streptococcus pneumoniae/pneumonia/kidney injury/neuraminidase/animal model/Dang-gui Shaoyao San分类
医药卫生引用本文复制引用
张春杰,韩文静,张小涵,余学龙,杨沫,王运来,许钒..加味当归芍药散对肺炎链球菌诱导幼鼠肾损伤的保护作用[J].中国药理学通报,2026,42(5):974-983,10.基金项目
国家自然科学基金资助项目(No 82274375) （No 82274375）
安徽省高校科研计划重大项目(No 2024AH040142) （No 2024AH040142）
