中华中医药杂志2026，Vol.41Issue(5)：1525-1529,5.
参芪地黄颗粒对局灶节段性肾小球硬化症大鼠足细胞损伤的保护作用及对HIF-1α、HMOX1、p27、PAI-1的影响
Protective effect of Shenqi Dihuang Granules on podocyte injury in focal segmental glomerulosclerosis rats and influence on HIF-1α,HMOX1,p27 and PAI-1
摘要
Abstract
Objective:To clarify the protective effect of Shenqi Dihuang Granules on focal segmental glomerulosclerosis(FSGS)podocyte injury,and to explore its underlying mechanism.Methods:Unilateral nephrectomy combined with adriamycin tail vein injection was used to construct a rat FSGS model,and 24-hour urinary protein quantification(24hUTP),serum plasma albumin(ALB),creatinine(Scr),urea nitrogen(BUN),total cholesterol(TC)and triglyceride(TG)levels were detected by colorimetry.PAS staining and electron microscopy were used to observe the pathological damage of the kidney.Western Blot was used to detect the expression of differentially expressed proteins.Results:Shenqi Dihuang Granules could alleviate the pathological damage of kidney,reduce 24hUTP(P＜0.01),increase the ALB level(P＜0.05),reduce the Scr,BUN,TC and TG levels(P＜0.01),increase the protein expression levels of PODXL,nephrin,HMOX1 and p27(P＜0.05),decrease the protein expression levels of HIF-1α and PAI-1(P＜0.05).Conclusion:Shenqi Dihuang Granules can repair podocyte damage and effectively improve the renal pathology of FSGS,and its mechanism is related to the regulation of podocyte-related proteins PODXL,nephrin and HIF-1α pathway-related molecules HIF-1α,HMOX1,p27 and PAI-1 proteins.关键词
局灶节段性肾小球硬化症/足细胞/参芪地黄颗粒/HIF-1α信号通路/中医药Key words
Focal segmental glomerulosclerosis(FSGS)/Podocytes/Shenqi Dihuang Granules/HIF-1α signaling pathway/Traditional Chinese medicine引用本文复制引用
郭婷,黄文龙,丁樱,宋纯东,高雅婵,白惠文,姜浩然,郑肖庆..参芪地黄颗粒对局灶节段性肾小球硬化症大鼠足细胞损伤的保护作用及对HIF-1α、HMOX1、p27、PAI-1的影响[J].中华中医药杂志,2026,41(5):1525-1529,5.基金项目
河南省自然科学基金项目(No.232300421306),河南省中医药科学研究专项课题(No.20-21ZY2167),河南省国家中医临床研究基地科研专项课题(No.2019JDZX2025,No.2022JDZX040),中国民族医药学会科研项目(No.2020ZY267-400601) （No.232300421306）
