中国医院用药评价与分析2026，Vol.26Issue(5)：539-544,6.DOI:10.14009/j.issn.1672-2124.2026.05.006
基于UPLC-MS/MS的临床验方愈疮散化学成分分析
Chemical Composition Analysis of Clinical Empirical Formula Yuchuang Powder Based on UPLC-MS/MS
摘要
Abstract
OBJECTIVE:To establish an ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)system for the comprehensive analysis of the chemical components of clinical empirical formula Yuchuang powder,to elucidate the fragmentation patterns of representative compounds,and to enhance the quality standards of traditional Chinese medicine preparations.METHODS:UPLC-MS/MS was employed to identify the chemical constituents in Yuchuang powder.The chromatographic separation was performed on an ACQUITY UPLC HSS T3 column(100 mm×2.1 mm,1.8 μm)with gradient elution using a mobile phase consisting of 0.1%formic acid in water and acetonitrile.The injection volume was 10 μL.Automated screening of chemical components was conducted bu using UNIFI software based on the UNIFI traditional Chinese medicine database.The identified compounds were classified and verified by comparison with public databases and published literature,accompanied by fragment ion matching.RESULTS:A total of 80 chemical components were identified,of which 58 were detected in positive ion mode,41 in negative ion mode,and 19 components were detected in both ion modes.Divided by compound categories,it mainly includes 39 terpenes,7 phenanthrene compounds,8 phenolic acids,4 alkaloids,as well as flavonoids,vitamin D derivatives,fatty acids,and other components.CONCLUSIONS:The UPLC-MS/MS method established in the study can identify the pharmacologically active chemical components and characteristic constituents of each medicinal material in Yuchuang powder,providing reference for the rapid identification and quality control of the chemical components of the preparation.关键词
愈疮散/超高效液相色谱-串联质谱法/化学成分/裂解规律Key words
Yuchuang powder/UPLC-MS/MS/Chemical constituents/Fragmentation pattern分类
医药卫生引用本文复制引用
黄雯琪,郭杨志,刘继业,刘敏,时正媛,段松冷..基于UPLC-MS/MS的临床验方愈疮散化学成分分析[J].中国医院用药评价与分析,2026,26(5):539-544,6.基金项目
首都卫生发展科研专项(No.首发2024-2-2087) （No.首发2024-2-2087）
北京市属医院科研培育计划(No.PZ2025005) （No.PZ2025005）
