中国中医急症2026，Vol.35Issue(5)：516-520,536,6.DOI:10.3969/j.issn.1004-745X.2026.05.004
黛矾散干预JAK2/STAT3/SOCS3信号通路保护原发性胆汁性胆管炎小鼠模型肝内胆管的研究
Study on Daifan Powder Intervening JAK2/STAT3/SOCS3 Signaling Pathway to Protect Intrahepatic Bile Ducts in Mouse Models of Primary Biliary Cholangitis
摘要
Abstract
Objective:To investigate the effect and mechanism of Daifan Powder in regulating JAK2/STAT3/SOCS3 signaling pathway on immune inflammatory response in primary biliary cholangitis(PBC).Methods:C57BL/6J mice were divided into the control group,model group,ursodeoxycholic acid group(100 mg/kg),and low-,medium-,and high-dose Daifan Powder groups(90,180,360 mg/kg).After preparing model of primary bili-ary cholangitis,all mice were given intragastric administration continuously for 28 days.Liver index of mice was measured;the levels of biochemical indicators including γ-GT,TBA,TBil,AST and ALT were detected by automat-ic biochemical analyzer;hematoxylin-eosin(HE)staining and Masson staining were used to observe histopathologi-cal changes of liver;the levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and interleukin-1 β(IL-1β)were detected by enzyme-linked immunosorbent assay(ELISA);the mRNA levels of JAK2,STAT3 and SOCS3 were detected by reverse transcription-polymerase chain reaction(RT-PCR);the levels of JAK2,p-JAK2,STAT3,p-STAT3 and SOCS3 were detected by Western blotting.Results:Compared with the control group,the liver index in the model group was significantly increased(P＜0.01);γ-GT,TBA,TBil,AST,ALT were elevated(P＜0.01);massive inflammatory cell infiltration and collagen fiber deposition were observed in the portal area of liver tissue;the expressions of TNF-α,IL-6 and IL-1 β were up-regulated(P＜0.01);the protein ratios of p-JAK2/JAK2,p-STAT3/STAT3 and the relative mRNA expressions of JAK2 and STAT3 in liver tissue were up-regulated(P＜0.01),while the protein and mRNA expressions of SOCS3 were down-regulated(P＜0.01).Compared with the model group,the above indicators in each treatment group were significantly improved(P＜0.05 or P＜0.01),and inflammation and collagen deposition in the portal area of liver tissue were alleviated.Conclusion:Daifan Powder can alleviate intrahepatic bile duct injury in PBC mice,which may be related to the regulation of inflamma-tory response mediated by JAK2/STAT3/SOCS3 signaling pathway.关键词
原发性胆汁性胆管炎/黛矾散/肝内胆管损伤/JAK2/STAT3/SOCS3信号通路/小鼠Key words
Primary biliary cholangitis/Daifan Powder/Intrahepatic bile duct injury/JAK2/STAT3/SOCS3 sig-naling pathway/Mice分类
医药卫生引用本文复制引用
计洋,徐子皓,徐俊,程良斌..黛矾散干预JAK2/STAT3/SOCS3信号通路保护原发性胆汁性胆管炎小鼠模型肝内胆管的研究[J].中国中医急症,2026,35(5):516-520,536,6.基金项目
国家自然科学基金面上项目(82274367) （82274367）
湖北省中医药管理局面上项目(ZY2025M007) （ZY2025M007）
湖北省时珍人才工程科研项目(鄂卫函[2024]256号) （鄂卫函[2024]256号）
