中国兽药杂志2026，Vol.60Issue(5)：24-30,7.DOI:10.11751/ISSN.1002-1280.2026.5.04
鹦鹉热衣原体数字PCR检测方法的建立与应用
Establishment and Application of a Digital PCR Detection Method for Chlamydia psittaci
摘要
Abstract
Chlamydia psittaci(C.psittaci)is a zoonotic pathogen that primarily causes respiratory tract infections.Due to the absence of specific clinical symptoms,it is prone to missed diagnosis and misdiagnosis,which may lead to severe illness,and its harmfulness is often underestimated.In this study,a pair of primers and a probe were designed based on the nucleotide sequence of the C.psittaci ompA gene(GenBank accession number:PQ511242.1)published in GenBank.A digital PCR(dPCR)detection method for C.psittaci was established,and the reaction system was optimized.The results showed that the optimal concentrations of primers and probe for dPCR were 900 nmol/L and 250 nmol/L,respectively,and the optimal annealing temperature was 56℃.The method achieved a lower limit of detection of 0.58 copies/μL,demonstrated a good linear relationship,and showed no cross-reaction with seven other pathogens,indicating high specificity and good reproducibility.In the clinical detection of 72 bovine and ovine blood and vaginal swab samples,the positive detection rate of dPCR was 19.04%,while that of real-time quantitative PCR(qPCR)was 18.06%.The dPCR method established in this study is more sensitive and efficient than the qPCR method and can be used for the quantitative detection of C.psittaci.关键词
鹦鹉热衣原体/数字PCR/检测方法Key words
Chlamydia psittaci/digital PCR/detection method分类
农业科技引用本文复制引用
梅浩,任小侠,孙伟峰,李俊平,王楠..鹦鹉热衣原体数字PCR检测方法的建立与应用[J].中国兽药杂志,2026,60(5):24-30,7.基金项目
国家重点研发项目"重大人兽共患病诊断标志物定值关键技术与标准物质研究"(2023YFF0724501) （2023YFF0724501）
