浙江大学学报（医学版）2026，Vol.55Issue(3)：222-233,12.DOI:10.3724/zdxbyxb-2025-0640
关节镇痛巴布膏对胶原诱导性关节炎大鼠抗炎作用的代谢组学研究
Metabolomics study on anti-inflammatory effects of Guanjie Zhentong Babu plaster in collagen-induced arthritis rats
摘要
Abstract
Objective:To investigate the anti-inflammatory effects of Guanjie Zhentong Babu plaster on collagen-induced arthritis(CIA)rats and its potential mechanisms.Methods:Rats were randomly divided into normal control group and experimental group.The experimental group was immunized with bovine type Ⅱ collagen emulsion to establish the CIA rat model,and then subdivided into the following groups:cold syndrome model group,heat syndrome model group,high-dose cold syndrome group,high-dose heat syndrome group,low-dose cold syndrome group,positive control cold syndrome group,positive control heat syndrome group,and blank plaster cold syndrome group,with eight rats in each group.Arthritis index(AI)scores of paw joints were evaluated on day 0,day 4,day 7,and day 14 after drug administration.A plethysmometer was used to assess hind paw joint swelling.After completion of the pharmacodynamic evaluation,the rats were sacrificed,and hematoxylin and eosin staining was performed to observe histopathological changes in ankle joint tissues.Serum metabolite profiles were analyzed using ultra performance liquid chromatography coupled with linear ion trap quadrupole-Orbitrap mass spectrometry,and metabolic pathway enrichment analysis was conducted to explore the potential mechanisms of drug intervention.In addition,immunohistochemistry and enzyme-linked immunosorbent assay were performed to investigate the levels of tumor necrosis factor-α(TNF-α),IL-17,and IL-10.Results:Compared with the cold syndrome model group,the high-dose cold syndrome group showed a significant improvement in AI scores on day 14(P<0.05)and a significant reduction in hind paw joint swelling(P<0.01),accompanied by markedly alleviated histopathological damage in the ankle joint.In contrast,no significant changes were observed in the high-dose heat syndrome group compared with the heat syndrome model group.Metabolomics analysis revealed that arginine biosynthesis and purine metabolism pathways were markedly disrupted in the cold-syndrome model group,and compared with the cold syndrome model group,the contents of L-citrulline were significantly increased,whereas the contents of uric acid and xanthine were significantly decreased in the high-dose cold syndrome group(all P<0.05).Immunohistochemistry and enzyme-linked immunosorbent assay results indicated that,compared with the cold syndrome model group,TNF-α and IL-17 levels in synovial tissue and serum were significantly decreased(all P<0.05),whereas IL-10 levels were significantly increased(all P<0.01)in the high-dose cold syndrome group.Conclusion:Guanjie Zhentong Babu plaster alleviates joint injury in CIA rats with cold syndrome by regulating arginine and purine metabolism-related pathways to restore inflammatory balance.关键词
关节镇痛巴布膏/炎性关节病/代谢组学/超高效液相色谱-质谱法/寒证/大鼠Key words
Guanjie Zhentong Babu Plaster/Inflammatory arthropathy/Metabolomics/Ultra performance liquid chromatography-mass spectrometry/Cold syndrome/Rats分类
医药卫生引用本文复制引用
徐天,徐金灵,杨小林,石荣,倪力强..关节镇痛巴布膏对胶原诱导性关节炎大鼠抗炎作用的代谢组学研究[J].浙江大学学报（医学版）,2026,55(3):222-233,12.基金项目
上海市中医药传承创新发展三年行动计划[ZY(2025-2027)-2-2-1]This study was supported by the Three-year Action Plan for Shanghai TCM Development and Inheritance Program[ZY(2025-2027)-2-2-1] （2025-2027）
