浙江大学学报（医学版）2026，Vol.55Issue(3)：267-277,11.DOI:10.3724/zdxbyxb-2025-0696
基于网络药理学策略预测并体外验证银杏内酯B的潜在适应证
Prediction and in vitro validation of potential indications for ginkgolide B based on network pharmacology strategies
摘要
Abstract
Objective:To predict potential indications for ginkgolide B(GB)based on network pharmacology strategies.Methods:GB targets were comprehensively identified by integrating data from the Therapeutic Target Database,the Comparative Toxicogenomics Database,and BindingDB,followed by further validation based on literature.A standardized disease-gene dataset was established by integrating data from the genome-wide association study and the Online Mendelian Inheritance in Man Database.The topological proximity between GB targets and disease modules within the human protein-protein interaction network was quantified using network proximity metrics,with statistical significance evaluated via Z-score and permutation testing(Z<0,P<0.05).Candidate diseases were classified according to MeSH terms,visualized through network mapping,and further characterized by gene set similarity based on the Jaccard index.Experiments in RAW 264.7 macrophages were conducted to validate the inhibitory effect of GB on nitric oxide and proinflammatory cytokines.Results:Twelve potential GB targets were identified,and a disease-gene dataset encompassing 680 diseases was established.Network proximity analysis revealed significant associations between GB and 22 diseases,of which 11 had prior literature support.Disease classification indicated that the predicted indications of GB were primarily enriched in immune-mediated inflammatory diseases,with notable examples including juvenile idiopathic arthritis(Z=-3.10)and Crohn disease(Z=-3.05).Network analysis suggested that GB may exert pleiotropic regulatory effects across multiple diseases via key targets such as MAPK1.The strongest gene set similarity was observed between juvenile idiopathic arthritis and ankylosing spondylitis(Jaccard index=0.60).In vitro experiments with macrophages demonstrated that GB suppresses the production of nitric oxide and proinflammatory cytokines,thereby supporting its potential as a therapeutic agent for immune-mediated inflammatory diseases.Conclusion:This study systematically predicted 22 potential indications for GB and confirmed its therapeutic potential for immune-mediated inflam-matory diseases through in vitro experiments.关键词
银杏内酯B/适应证/系统生物学/网络药理学/网络邻近度/药物重定位/免疫介导炎症性疾病Key words
Ginkgolide B/Indication/System biology/Network pharmacology/Network proximity/Drug repositioning/Immune-mediated inflammatory disease分类
医药卫生引用本文复制引用
张慧莹,范庆,张轶骅,刘梦晨,崔国祯..基于网络药理学策略预测并体外验证银杏内酯B的潜在适应证[J].浙江大学学报（医学版）,2026,55(3):267-277,11.基金项目
国家自然科学基金(82160684) （82160684）
贵州省科技计划(黔科合基础-ZK[2022]一般587) （黔科合基础-ZK[2022]一般587）
广东省普通高校创新团队(2024KCXTD005)This study was supported by National Natural Science Foundation of China(82160684),Guizhou Provincial Science and Technology Plan(Qian Science Joint Basic-ZK[2022]General 587)and Innovation Team Project by Department of Education of Guangdong Province(2024KCXTD005) （2024KCXTD005）
