广东海洋大学学报2026，Vol.46Issue(3)：65-73,9.DOI:10.3969/j.issn.1673-9159.2026.03.007
尼罗罗非鱼IgM抗体免疫应答水平与抗病能力的关联性及遗传力评估
Correlation Between Level of IgM Antibody Immune Response and Disease Resistance in Nile tilapia,and Heritability Evaluation
摘要
Abstract
[Objective]This study aims to elucidate the correlation between specific IgM antibody levels and resistance to Streptococcus agalactiae infection in tilapia(Oreochromis niloticus)after immunization,and estimate the genetic parameters of this disease resistance trait,and evaluate its poten-tial as an indicator for disease-resistant breeding.[Method]Three populations of tilapia(Huadu,Panyu,and Hainan populations)were immunized by immersion with an inactivated S.agalactiae vac-cine,followed by a booster immunization at 15 days post-initial immunization(dpi).The levels of serum-specific IgM antibody were detected prior to immunization(0 d)and at 7,14,21,28,60,and 90 dpi to reveal its temporal dynamics.The correlations between the peak levels of serum-specific IgM antibody and relative percent survival(RPS),survival status and blood bacterial load following artifi-cial challenge with S.agalactiae were analyzed.Multiple full-sib families were constructed,and serum-specific IgM antibody levels of offspring from each family after immunization were measured.The vari-ance components and heritability were estimated using restricted maximum likelihood(REML),and the individual breeding values were predicted by best linear unbiased prediction(BLUP).[Result]The serum-specific IgM antibody levels in tilapia peaked(from 68.07 to 91.73 μg/mL)at 7 days after the second immunization and persisted at a high mass concentration(>54.99 μg/mL)for 28 days.Tilapia populations with higher post-immunization IgM antibody levels exhibited higher relative percent sur-vival(RPS).Surviving individuals after the bacterial challenge showed significantly higher IgM anti-body response levels than those that died(P<0.05),and individuals with higher IgM antibody response levels had a longer survival time after the challenge.In non-immunized tilapia subjected to bacterial challenge,serum-specific IgM levels were extremely significantly negatively correlated with blood S.agalactiae load(P<0.01).Genetic analysis based on 24 full-sib families revealed that the heritability of the IgM antibody immune response trait in tilapia was 0.44.[Conclusion]IgM antibody immune response level in tilapia is positively correlated with its resistance to S.agalactiae infection and repre-sents a highly heritable trait.The IgM antibody immune response level can serve as an important candi-date trait for disease-resistant breeding in tilapia,providing a theoretical foundation for developing new strains with enhanced immunocompetence and disease resistance.关键词
尼罗罗非鱼/免疫应答/IgM抗体/抗病性状/遗传力Key words
Oreochromis niloticus/immune response/IgM antibody/disease resistance trait/heritability分类
农业科技引用本文复制引用
刘洪,刘志刚,黄少尾,王章,陈善真,衣萌萌,王淼,于浩东,卢迈新,可小丽..尼罗罗非鱼IgM抗体免疫应答水平与抗病能力的关联性及遗传力评估[J].广东海洋大学学报,2026,46(3):65-73,9.基金项目
中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金(2024SJRC2) （2024SJRC2）
国家现代农业产业技术体系项目(CARS-46) （CARS-46）
2022年水产种业体系建设—罗非鱼"育繁推"一体化育种科企联合体项目(粤财农[2022]119号) （粤财农[2022]119号）
