广东海洋大学学报2026，Vol.46Issue(3)：164-174,11.DOI:10.3969/j.issn.1673-9159.2026.03.017
黄鳝养殖废液中活性肽筛选及其抗氧化应激与皮肤愈合活性
Screening of Bioactive Peptides from Aquaculture Wastewater of Monopterus albus and Their Activities in Antioxidant Stress and Skin Wound Healing
摘要
Abstract
[Objective]This study aims to explore the utilization value of bioactive peptides derived from the aquaculture wastewater of Asian swamp eel(Monopterus albus),screen candidate peptides with antioxidant and cell repair functions,and provide a theoretical basis for their application in novel biomaterials.[Methods]Using eel aquaculture wastewater as raw material,peptide mixtures were prepared via physical enrichment and pepsin hydrolysis.Candidate peptides were screened based on bioactivity,water solubility,toxicity and allergenicity,and their binding modes with the Keap1 protein were evaluated via molecular docking.A lipopolysaccharide(LPS)-induced oxidative stress model was established using mouse monocyte macrophages(RAW264.7)to detect the intracellular levels of reactive oxygen species(ROS),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and malondialdehyde(MDA).Furthermore,a scratch wound model in human skin fibroblasts(HSF)was utilized to evaluate the effects of the active peptides on cell migration.[Results]The mass fraction of protein in the lyophilized powder of the wastewater was 3.12%.The enzymatic hydrolysates were mainly small-molecule peptides with 4 to 15 amino acid residues,which were rich in hydrophobic and partially aromatic amino acids.Five non-toxic candidate wastewater-derived peptides with good water solubility were obtained through multi-dimensional screening,among which the sequence FEGPPFKFKFTLR(hereafter referred to as FEG-13)exhibited the lowest binding free energy with Keap1 and formed a stable complex conformation.Cytological evaluation demonstrated that FEG-13 intervention could significantly decrease the intracellular ROS level in LPS-induced RAW264.7 cells by 48.7%(P<0.001),significantly reduce the MDA molality to 5.23 nmol/mg(P<0.01),and significantly restore the activities of SOD and GSH-Px to 12.77 and 23.12 U/mg(P<0.01),respectively.Meanwhile,intervention with 1.0 mg/mL FEG-13 significantly enhanced the scratch closure rate of HSF cells to 64.43%(P<0.001).[Conclusion]Eel aquaculture wastewater holds potential to obtain various bioactive peptides.The obtained FEG-13 can alleviate cellular oxidative stress by regulating the Keap1-Nrf2 pathway and drive fibroblast migration,exhibiting outstanding potential for antioxidant activity and cell repair.关键词
黄鳝养殖废液/生物活性肽/抗氧化应激/皮肤愈合/气浮法/分子对接Key words
eel aquaculture wastewater/bioactive peptides/anti-oxidative stress/skin healing/dissolved air flotation/molecular docking分类
资源环境引用本文复制引用
钟菱,邱亮,廖涛,白婵,赵忠涛,印杰,陈小奇,金士威..黄鳝养殖废液中活性肽筛选及其抗氧化应激与皮肤愈合活性[J].广东海洋大学学报,2026,46(3):164-174,11.基金项目
仙桃市黄鳝产业高质量发展中心基金(202412003) （202412003）
湖北省重点研发计划项目(2023BCB113) （2023BCB113）
