浙江农业学报2026，Vol.38Issue(5)：857-866,10.DOI:10.3969/j.issn.1004-1524.20250307
三角鲂胚胎不同发育时期的代谢组分析
Metabolome analysis of embryos of Megalobrama terminalis at different developmental stages
摘要
Abstract
To investigate the types,dynamic variation patterns and regulatory metabolic pathways of metabolites dur-ing different developmental stages of Megalobrama terminalis embryos,a non-targeted metabolomic approach based on liquid chromatography-mass spectrometry(LC-MS)was employed to analyze embryo samples collected at the gas-trula stage(17 h),neurula stage(25 h),somitogenesis stage(32 h),tail bud stage(41 h),muscular effect stage(50 h)and heart beating stage(58 h).The results showed that a total of 952 metabolites were detected,mainly in-cluding lipids and lipid-like molecules,organic acids and derivatives,and organoheterocyclic compounds.Compara-tive analysis of differential metabolites revealed that 9,11-tetradecadienedioylcarnitine and paraldehyde were unique to Megalobrama terminalis embryos at 32 h of development,while sepiapterin,stearaldehyde and β-nitropropionic acid were specific metabolites at 58 h.KEGG enrichment analysis indicated that metabolic pathways such as nucleo-tide metabolism,ABC transporters and aminoacyl-tRNA biosynthesis exerted important biological functions in the processes of genetic material and protein biosynthesis,energy metabolism and signal transduction during embryonic development of Megalobrama terminalis.The findings of this study can provide theoretical support for improving the hatching rate of fertilized eggs of Megalobrama terminalis.关键词
三角鲂/胚胎发育/代谢组学/差异代谢物/高效孵化Key words
Megalobrama terminalis/embryonic development/metabolomics/differential metabolites/high-efficien-cy hatching分类
农业科技引用本文复制引用
于咏清,唐红,简杰亮,顾志敏,关文志..三角鲂胚胎不同发育时期的代谢组分析[J].浙江农业学报,2026,38(5):857-866,10.基金项目
国家重点研发计划(2022YFD2400605) （2022YFD2400605）
