浙江中医药大学学报2026，Vol.50Issue(4)：391-404,14.DOI:10.16466/j.issn1005-5509.2026.04.001
基于网络药理学和动物实验探讨升陷汤促进膀胱逼尿肌细胞增殖的作用机制
Exploring the Mechanism of Shengxian Decoction in Promoting Detrusor Muscle Cells Proliferation Based on Network Pharmacology and Animal Experiments
摘要
Abstract
[Objective]To investigate the therapeutic effect and mechanism of Shengxian Decoction on bladder detrusor injury.[Methods]Based on the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),the active components and drug targets of Shengshen Decoction were screened;the related targets of urinary retention were obtained from GeneCards,Therapeutic Target Database(TTD)and DrugBank database.The"traditional Chinese medicine-active component-target"network and protein-protein interaction(PPI)network were constructed to screen the core targets.Gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis were performed to construct a"pathway-target-component"network.Molecular docking was employed to verify the binding ability of core components to their targets.Through bladder neck and urethra ligation,a rat model of partial bladder outlet obstruction(pBOO)was established.After modeling,the rats were randomly divided into sham operation group(Sham),model group(Mol),and Shengxian Decoction low(SXTL,3.9 g·kg-1),medium(SXTM,7.8 g·kg-1),high dose group(SXTH,15.6 g·kg-1),western medicine group[Tamsulosin-Pyridostigmine Bromide(TS-PB),Pyridosine Bromide(18 mg·kg-1)combined with Tamsulosin capsule contents(0.02 mg·kg-1)].After 4 weeks of intervention,the bladder mass index,histomorphology,cell proliferation,mRNA and protein expression of cyclic adenosine monophosphate(cAMP),protein kinase A(PKA),and cAMP-response element binding protein(CREB)were detected.[Results]A total of 1 117 active ingredients of Shengxian Decoction,233 targets of action and 210 intersection targets of urinary retention were selected.The core targets included tumor protein 53(TP53),protein kinase B(AKT1)and Jun kinase(JNK).KEGG enrichment analysis showed that target genes were mainly enriched in the following signaling pathways:phosphoinositide 3-kinase/protein kinase B signaling pathway(PI3K/AKT),mitogen-activated protein kinases(MAPK),hypoxia inducible factor-1(HIF-1)and cAMP signaling pathways.The core active ingredients were quercetin,kaempferol,luteolin,7-O-methylisomucinol,and trichomycin.Shengxian Decoction treatment significantly reduced the bladder weight/body weight ratio(P<0.001,P<0.0001),reduced collagen fiber deposition(P<0.0001),down-regulated the mRNA expression of cAMP,PKA and CREB(P<0.01,P<0.001,P<0.0001),and inhibited the protion expression of cAMP,p-PKA/PKA and p-CREB/CREB(P<0.05,P<0.01,P<0.0001),SXTH showed particularty good effect(P<0.0001).[Conclusion]Shengxian Decoction can inhibit the cAMP signaling pathway,alleviate bladder fibrosis and promote the proliferation of bladder detrusor muscle cells through the synergistic action of multi-components,multi-targets and multi-pathways,so as to improve bladder structure and function after pBOO.关键词
膀胱出口梗阻/癃闭/升陷汤/逼尿肌细胞/增殖/网络药理学/cAMP/EdUKey words
bladder outlet obstruction/urinary retention/Shengxian Decoction/detrusor muscle cells/proliferation/network pharmacology/cAMP/EdU分类
医药卫生引用本文复制引用
付亚萍,周琳,陶方泽,杨海甜,田艺璨..基于网络药理学和动物实验探讨升陷汤促进膀胱逼尿肌细胞增殖的作用机制[J].浙江中医药大学学报,2026,50(4):391-404,14.基金项目
南京市中医药青年人才培养计划项目(ZYQ20041) （ZYQ20041）
第七批全国老中医药专家学术经验继承工作项目(国中医药人教函[2022]76号) （国中医药人教函[2022]76号）
江苏省研究生科研与实践创新计划项目(SJCX23_0814、SJCX25_1063、SJCX25_1067) （SJCX23_0814、SJCX25_1063、SJCX25_1067）
第五批江苏省中医临床优秀人才研修项目(苏中医科教[2025]9号) （苏中医科教[2025]9号）
南京市(赵薇)名中医工作室建设项目(宁卫中医[2023]9号)Nanjing TCM Young Talents Training Project(ZYQ20041) （赵薇）
The Seventh Batch of National Chinese Medicine Experts Academic Experience Inheritance Project(G.TCM.R.J.H.[2022]76) （G.TCM.R.J.H.[2022]76）
Jiangsu Graduate Research and Practice Innovation Project(SJCX23_0814,SJCX25_1063,SJCX25_1067) （SJCX23_0814,SJCX25_1063,SJCX25_1067）
The Fifth Batch of TCM Clinical Excellent Talents Training Project in Jiangsu Province(S.TCM.K.J.[2025]9) （S.TCM.K.J.[2025]9）
Nanjing(ZHAO Wei)Famous Traditional Chinese Medicine Doctor Studio Construction Project(N.W.TCM.[2023]9) （ZHAO Wei）
