作物学报2026，Vol.52Issue(6)：1593-1603,11.DOI:10.3724/SP.J.1006.2026.51106
小麦Pod-A1、Pod-D1和Pod-2D位点等位变异对籽粒过氧化物酶活性的遗传效应分析
Analysis of the genetic effects of allelic variation at the Pod-A1,Pod-D1,and Pod-2D loci on peroxidase activity in wheat grains
摘要
Abstract
Peroxidase(POD)activity in wheat grains strongly influences processing quality and the color of wheat-based products,and is therefore an important target for quality improvement.In this study,151 wheat varieties(lines)from domestic and international sources were used to systematically characterize allelic variation at the Pod-A1,Pod-D1,and Pod-2D loci using molecular markers.Together with multi-year,multi-location measurements of grain POD activity,we evaluated the effects of individual alleles and their combinations on POD activity.Grain POD activity showed substantial genetic variation,with a mean of 674.39 U g-1 min-1,a range of 431.30-954.81 U g-1 min-1,and a coefficient of variation of 15.52%.Environ-ment,genotype,and their interaction all had highly significant effects on POD activity(P<0.01).All three loci significantly affected grain POD activity,and the favorable alleles were Pod-A1b,Pod-D1b,and Pod-2D-GG,respectively.In total,12 alle-lic combinations were identified and classified into high-,medium-,and low-activity combination types based on grain POD activity.POD activity differed significantly among the three types(P<0.05),whereas no significant differences were detected within each type.The genetic effects of the three loci on grain POD activity followed the order Pod-2D>Pod-A1>Pod-D1.Grain POD activity increased significantly with the number of favorable alleles(R2=0.9681,P<0.05);varieties(lines)pyramiding 2-3 favorable alleles had significantly higher POD activity than those carrying 0-1 favorable allele(P<0.05).Significant regional differences were observed in POD activity,allelic variation,and allelic-combination frequencies.Nine varieties,including Liangxing 66,Lankao 24,and Jimai 22,were identified as carrying all three favorable alleles and exhibi-ting POD activity above 800 U g-1 min-1.These findings provide a theoretical basis and germplasm resources for molecular marker-assisted breeding to optimize wheat grain POD activity.关键词
小麦/加工品质/面制品颜色/过氧化物酶/POD基因/分子标记辅助育种Key words
Triticum aestivum/processing quality/wheat-based product color/peroxidase/POD genes/molecular marker-assisted breeding引用本文复制引用
翟胜男,王颖,耿洪伟,刘建军,曹新有,李豪圣,李吉虎,李法计,刘金栋,夏先春,吕莹莹,马瑞峰..小麦Pod-A1、Pod-D1和Pod-2D位点等位变异对籽粒过氧化物酶活性的遗传效应分析[J].作物学报,2026,52(6):1593-1603,11.基金项目
本研究由山东省重点研发计划项目(2023CXPT079-2),山东省自然科学基金项目(ZR2023MC205 和 ZR2023MC155),泰山学者工程项目(tspd20221108 和tsqn202408304),济南市"新高校 20 条"资助项目(202228067)和财政部和农业农村部国家现代农业产业技术体系建设专项(小麦,CARS-03-06)资助.This study was supported by the Key R&D Program of Shandong Province(2023CXPT079-2),the Natural Science Foundation of Shandong Province(ZR2023MC205 and ZR2023MC155),the Taishan Scholar Program(tspd20221108 and tsqn202408304),the Funding Program of"20 New Policies for Higher Education Institutions"of Jinan City(202228067),and the China Agriculture Research System of MOF and MARA(Wheat,CARS-03-06). （2023CXPT079-2）
