作物学报2026，Vol.52Issue(6)：1618-1630,13.DOI:10.3724/SP.J.1006.2026.54151
甘蔗类四跨膜蛋白ScTSPAN18与6K2互作应答SCMV侵染研究
Interaction between the sugarcane tetraspanin-like protein ScTSPAN18 and 6K2 in response to SCMV infection
摘要
Abstract
Tetraspanins(TETs)are a conserved class of transmembrane proteins that are widely distributed across cellular orga-nisms and play important roles.TETs function in part by assembling tetraspanin-enriched microdomains(TEMs).In our previous study,we screened and identified a TET-like protein,ScTSPAN18,from a sugarcane cDNA yeast library using the 6K2 protein of sugarcane mosaic virus(SCMV)as bait,and we also characterized the TET gene family in the sugarcane(Saccharum spp.hybrid)cultivar Xintaitang 22(XTT22).Here,the interaction between SCMV-6K2 and ScTSPAN18 was further validated using bimol-ecular fluorescence complementation(BiFC)and luciferase complementation assay(LCA).Bioinformatic analyses showed that ScTSPAN18 is 251 amino acids long and comprises four transmembrane domains.Structural modeling indicated that ScTSPAN18 differs from classical TETs,and yeast two-hybrid(Y2H)assays showed that ScTSPAN18 neither self-interacts nor interacts with classical TETs.Phylogenetic analysis suggested that TET like proteins are divergent between monocotyledonous and dicotyledo-nous species,as well as between C3 and C4 plants.Subcellular localization assays indicated that ScTSPAN18 is localized to the endoplasmic reticulum.RT-qPCR analysis showed that ScTSPAN18 exhibits tissue-specific expression,with relatively high transcript levels in leaves and roots;expression in the+1 leaf was significantly higher than that in the 3rd and 8th internodes.Following SCMV challenge,ScTSPAN18 expression increased significantly at early infection stages and remained high until day 14,after which it decreased to the basal level.关键词
甘蔗花叶病毒/6K2/四跨膜蛋白/类四跨膜蛋白/蛋白互作Key words
sugarcane mosaic virus/6K2/tetraspanin/tetraspanin-like protein/protein interaction引用本文复制引用
崔致远,秦晨展,刘星雨,张海,曾康,黄国强,徐景升..甘蔗类四跨膜蛋白ScTSPAN18与6K2互作应答SCMV侵染研究[J].作物学报,2026,52(6):1618-1630,13.基金项目
本研究由国家重点研发计划项目(2023YFD1200703)和福建农林大学科技创新基金项目(KFB24033)资助.This study was supported by the National Key Research and Development Program of China(2023YFD1200703)and the Science and Tech-nology Innovation Project of Fujian Agriculture and Forestry University(KFB24033). （2023YFD1200703）
