作物学报2026，Vol.52Issue(6)：1631-1645,15.DOI:10.3724/SP.J.1006.2026.54135
耐弱光马铃薯品种筛选及转录因子编码基因StPIF3的克隆与功能分析
Screening of low-light-tolerant potato varieties and cloning and functional analysis of the transcription factor gene StPIF3
摘要
Abstract
Based on measurements and comparisons of growth traits,chlorophyll fluorescence parameters,and other related indi-ces in nine potato varieties under low-light treatment,the cultivar"Atlantic"was identified as low-light tolerant by principal component analysis.To investigate genes involved in the low-light response of"Atlantic",we performed transcriptome analysis after treatment with a light intensity of 10 μmol m-2 s-1 for 48 h.Using GO enrichment,KEGG pathway analysis,and transcrip-tion factor prediction,we identified the light-responsive transcription factor gene StPIF3 and cloned its full-length cDNA.StPIF3 was introduced into tobacco via agrobacterium-mediated transformation,and T1 transgenic lines were obtained by selfing.Bioin-formatics analysis,RT-qPCR,and chlorophyll fluorescence measurements were used to characterize and validate its function.StPIF3 encodes a 716-amino-acid protein with a predicted molecular weight of 76.77 kD and an isoelectric point of 7.26,and it is predicted to be hydrophilic and unstable.The StPIF3 protein contains a typical HLH domain and belongs to the bHLH family;phylogenetic analysis indicated that StPIF3 is most closely related to the tobacco homolog.Under 50 μmol m-2 s-1 light intensity,transgenic tobacco plants showed significantly higher Fv/Fm,qP,ETR,ETRmax,and SPAD values than wild-type plants,whereas F0 was lower.Overall,overexpression of StPIF3 markedly improves electron transport and light energy use efficiency,thereby enhancing photosynthetic performance and low-light tolerance.关键词
马铃薯/弱光耐性/StPIF3转录因子/bHLH/过表达Key words
Solanum tuberosum/weak light tolerance/transcription factors StPIF3/bHLH/overexpression引用本文复制引用
王文辕,燕雪嘉,刘玉霖,孙晓彤,李亚楠,唐鑫华,石瑛..耐弱光马铃薯品种筛选及转录因子编码基因StPIF3的克隆与功能分析[J].作物学报,2026,52(6):1631-1645,15.基金项目
本研究由财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-09)资助.This study was supported by the China Agriculture Research System of MOF and MARA(CARS-09). （CARS-09）
