花生籽仁总超长链脂肪酸和7种脂肪酸组分的QTL定位
QTL mapping of total very long-chain fatty acids and seven fatty acid components in peanut seeds
摘要
Abstract
Peanut oil quality is largely determined by its fatty acid composition;therefore,improving the fatty acid profile is a key objective in peanut breeding.Here,we used a recombinant inbred line(RIL)population derived from the cross Jihuatian 1×PI478819 to map quantitative trait loci(QTLs)for total very long-chain fatty acids(TVLCFAs)and seven individual fatty acid components across two environments,using a combined strategy of BSA-seq and conventional linkage mapping.Based on the major QTLs,we developed kompetitive allele-specific PCR(KASP)markers and validated them in the RIL population.In total,seven QTLs associated with TVLCFAs and the seven fatty acid components were identified.Three QTLs(qA06.1,qA08.1,and qA16.1)were consistently detected in both environments and co-localized for TVLCFAs and behenic acid content,explaining 7.10%-32.85%of the phenotypic variance(PVE).Notably,qA08.1 was also associated with oleic acid,linoleic acid,and palmitic acid contents in both environments,with PVE values of 13.73%-29.33%.The markers Tif2.A06.115805462,Tif2.A08.45741511,and Tif2.A16.148167815,developed for these three major QTLs,effectively distinguished lines with contrasting levels of TVLCFAs,arachidic acid,and behenic acid.In addition,Tif2.A08.45741511 was closely associated with oleic acid,linoleic acid,and palmitic acid contents.Overall,these results provide useful genetic resources for breeding peanuts with low TVLCFAs and high oleic acid content and support accelerated marker-assisted selection for high-quality specialty peanut cultivars.关键词
花生/脂肪酸组分/总超长链脂肪酸/集群分离体分析测序/QTL定位Key words
peanut/fatty acid composition/total very long-chain fatty acids/BSA-seq/QTL mapping引用本文复制引用
郑玉珍,徐静,苗利娟,黄冰艳,董文召,郑峥,张新友,齐飞艳,孙子淇,刘华,秦利,石磊,王娟,汪蒙蒙,韩锁义..花生籽仁总超长链脂肪酸和7种脂肪酸组分的QTL定位[J].作物学报,2026,52(6):1646-1657,12.基金项目
本研究由辽宁省粮食储藏种质创新专项(2023YYJH1/10200002),国家重点研发计划项目(2023YFD1202800),河南省科技项目(232102110234),财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-13),河南省现代农业产业技术体系(HARS-22-05-S),河南省农业科学院杰出青年科技基金(2024JQ06)和河南省农业科学院自主创新项目(2025ZC13)资助.This study was supported by the Liaoning Province Specialized Program for Grain Storage Germplasm Innovation(2023YYJH1/10200002),the National Key Research and Development Program of China(2023YFD1202800),the Science and Technology Project of Henan Province(232102110234),the China Agriculture Research System of MOF and MARA(CARS-13),the Henan Provincial Agriculture Research System,China(HARS-22-05-S),the Fund for Distinguished Young Scholars from Henan Academy of Agricultural Sciences(2024JQ06),and the Independent Innovation Project of the Henan Academy of Agricultural Sciences(2025ZC13). （2023YYJH1/10200002）
