作物学报2026，Vol.52Issue(6)：1711-1727,17.DOI:10.3724/SP.J.1006.2026.51096
小麦ABHD6基因家族鉴定与粒重功能标记开发
Identification of the ABHD6 gene family and development of functional markers for grain weight in wheat
摘要
Abstract
α/β-Hydrolase domain-containing protein 6(ABHD6),a key member of the serine hydrolase superfamily,plays impor-tant roles in regulating plant growth,development,and responses to environmental stress.However,a systematic genome-wide identification and analysis of the ABHD6 gene family in wheat(Triticum aestivum L.)is still lacking.Here,we integrated phylogenetic analysis,protein sequence characterization,promoter cis-acting element prediction,and haplotype analysis,and identified 18 TaABHD6 family members in the wheat genome.Phylogenetic and structural analyses showed that TaABHD6 pro-teins are closely related to rice OsABHD6 proteins,and that members within the same clade share highly conserved domains.Promoter analysis revealed that TaABHD6 promoters are enriched in cis-acting elements associated with plant growth and devel-opment.By analyzing polymorphisms in TaABHD6 genes and their associations with grain phenotypes,we identified eight genes with distinct haplotypes.Haplotype variation in three genes was significantly associated with grain-related traits;in particular,TaABHD6-5 haplotypes were significantly associated with grain weight,length,width,and thickness.We developed a KASP marker based on an A/T SNP at-1045 bp in the TaABHD6-5 promoter.Validation in 305 wheat accessions showed that this func-tional marker effectively distinguished two haplotypes:268 accessions carried TaABHD6-5-HapI,34 carried TaABHD6-5-HapII,two were heterozygous,and one accession failed genotyping.Association analysis further indicated that TaABHD6-5-HapI is a favorable haplotype for increased grain weight.qRT-PCR showed that TaABHD6-5 was relatively highly expressed in stems and spikes at booting and during early grain development;notably,expression was significantly lower in the favorable haplotype TaABHD6-5-HapI than in TaABHD6-5-HapII,suggesting that TaABHD6-5 may act as a negative regulator of grain weight.Se-quence analysis further suggested that TaABHD6-5 is targeted by miR160.Together,these results provide genome-wide evidence for the roles of TaABHD6 genes in wheat grain development and highlight the potential breeding value of superior haplotypes.关键词
小麦/α/β-水解酶结构域蛋白6(ABHD6)/基因家族鉴定/单倍型/分子标记Key words
wheat/α/β-hydrolase domain-containing protein 6(ABHD6)/gene family identification/haplotype/molecular marker引用本文复制引用
张献丰,郭利建,李康春,孔斌雪,刘玉芳,车卓,杨德龙..小麦ABHD6基因家族鉴定与粒重功能标记开发[J].作物学报,2026,52(6):1711-1727,17.基金项目
本研究由甘肃省科技重大专项(25ZDNA001),甘肃省麦类产业技术体系项目(GSARS07),甘肃省教育厅青年博士支持项目(2024QB-062)和省部共建干旱生境作物学国家重点实验室开放基金项目(GSCS-2023-07)资助.This study was supported by the Gansu Provincial Science and Technology Major Project(25ZDNA001),the Gansu Province Wheat Industry Technology System Project(GSARS07),the Youth Doctoral Support Project of Gansu Provincial Department of Education(2024QB-062),and the Open Fund of the State Key Laboratory of Cropology in Arid Habitat Jointly Established by the Province and Ministry(GSCS-2023-07). （25ZDNA001）
