作物学报2026，Vol.52Issue(6)：1859-1875,17.DOI:10.3724/SP.J.1006.2026.51086
水培盐碱复合胁迫下'济麦60'苗期根系有机酸分泌动态变化及其转录调控机制
Dynamic changes in root organic acid secretion and its transcriptional regulatory mechanisms in'Jimai 60'seedlings under combined salinity-alkalinity stress in hydroponics
摘要
Abstract
Soil salinization is a major constraint on global agricultural production,substantially reducing wheat yield and threat-ening food security.Root secretion of organic acids is an important physiological strategy by which plants cope with abiotic stress.However,under combined salt-alkali stress,the temporal dynamics of organic acid secretion in wheat roots and the underlying transcriptional regulatory networks remain poorly understood.Here,the salt-alkali-tolerant wheat cultivar'Jimai 60'was used to systematically characterize time-resolved metabolomic and transcriptomic profiles of root organic acids under combined salt-alkali stress,and key regulatory modules were identified using WGCNA.Combined salt-alkali stress markedly reshaped the composition and abundance of root organic acids.Across four comparison groups,44 differentially accumulated organic acids were identified,including 12 that were consistently upregulated and 4 that were consistently downregulated.Transcriptome analy-sis showed that the number of differentially expressed genes increased progressively with longer stress exposure.Genes related to photosynthesis,sexual reproduction,and porphyrin metabolism were persistently activated,whereas cell wall organization and oxidative phosphorylation exhibited stage-specific responses.WGCNA further identified the MEblue,MEturquoise,and MEyel-low modules as significantly associated with major organic acid metabolic traits;genes in these modules were mainly enriched in pathways related to energy metabolism,amino acid biosynthesis,and redox homeostasis.Overall,this study delineates the dy-namic landscape of root organic acid metabolism and transcriptional regulation in salt-alkali-tolerant wheat('Jimai 60')under combined salt-alkali stress.These findings advance our understanding of the physiological basis of wheat salt-alkali tolerance and provide a foundation,as well as candidate gene resources,for molecular breeding aimed at improving root function and stress resilience.关键词
小麦/盐碱复合胁迫/根系/有机酸/转录组/加权基因共表达网络分析Key words
wheat/combined saline-alkaline stress/roots/organic acids/transcriptome/WGCNA引用本文复制引用
陈雪燕,吕胜源,吴印莹,赵振东,曹新有,万何平,何华川,李政嘉,董新盼,李藕琪,刘小云,李丹萍,陈志伟,刘国霞..水培盐碱复合胁迫下'济麦60'苗期根系有机酸分泌动态变化及其转录调控机制[J].作物学报,2026,52(6):1859-1875,17.基金项目
本研究由财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-03-06),山东省重点研发计划项目(2023LZGC009,2024LZGCQY001)和山东省泰山学者工程项目(tstp20240843)资助.This study was supported by the China Agriculture Research System of MOF and MARA(CARS-03-06),the Key Research and Development Program of Shandong Province(2023LZGC009,2024LZGCQY001),and the Taishan Scholar Program(tstp20240843). （CARS-03-06）
