作物学报2026，Vol.52Issue(6)：1902-1912,11.DOI:10.3724/SP.J.1006.2026.52044
水稻SPX基因家族鉴定及响应磷处理的表达分析
Genome-wide identification and expression analysis of the SPX gene family in rice under phosphorus treatment
摘要
Abstract
This study aimed to systematically identify the rice SPX gene family at the whole-genome level,characterize its evolu-tionary features and expression patterns,and provide a basis for elucidating SPX functions in phosphorus signal transduction.Bioinformatics approaches were used to identify SPX genes in rice and to analyze their physicochemical properties,phylogenetic relationships,gene structures,conserved motifs,promoter cis-acting elements,and collinearity.Transcriptomic data and quantita-tive real-time PCR(RT-qPCR)were used to examine tissue-specific expression and responses to phosphorus stress.Six SPX genes were identified and mapped to five chromosomes.Phylogenetic analysis showed that OsSPX1 and OsSPX2 clustered together with 100%bootstrap support,whereas OsSPX3 formed a well-supported subclade with OsSPX5 and OsSPX6.Collinearity analy-sis identified two segmental duplication pairs(OsSPX1/OsSPX2 and OsSPX5/OsSPX6),and all Ka/Ks values were<1(0.19-0.44),indicating strong purifying selection.Gene structure analysis showed that OsSPX members contain 2-3 exons,and conserved motif distributions were highly consistent with the phylogenetic relationships.Promoter analysis identified 135 cis-acting ele-ments,mainly related to hormone,light,and stress responses,with methyl jasmonate-and abscisic acid-responsive elements being the most abundant.RT-qPCR analysis in the ShijinB variety indicated that OsSPX1-OsSPX6 were preferentially expressed in roots,with OsSPX3 also showing relatively high expression in inflorescences and OsSPX1 and OsSPX4 showing relatively high expression in leaves.Under low-phosphorus conditions,OsSPX1,OsSPX2,OsSPX3,OsSPX5,and OsSPX6 were significantly upregulated,with OsSPX3 showing the strongest induction,whereas OsSPX4 was not affected by phosphorus availability.Overall,the rice SPX gene family appears to have expanded via segmental duplication and subsequently evolved under purifying selection;although gene structures and motif compositions are relatively conserved,members show clear tissue specificity and differential responsiveness to phosphorus stress.关键词
水稻/SPX基因家族/磷胁迫/生物信息学/表达分析Key words
rice/SPX gene family/phosphorus stress/bioinformatics/expression analysis引用本文复制引用
胡赵,钱润,谢丰璞,应素平..水稻SPX基因家族鉴定及响应磷处理的表达分析[J].作物学报,2026,52(6):1902-1912,11.基金项目
本研究由山西省基础研究计划项目(202503021212246)资助.This study was supported by the Fundamental Research Program of Shanxi Province(202503021212246). （202503021212246）
