植物保护2026，Vol.52Issue(3)：143-151,9.DOI:10.16688/j.zwbh.2025303
大豆双生病毒B丹参分离物的基因组结构分析及遗传变异研究
Genomic structure analysis and genetic variation of soybean geminivirus B Salvia miltiorrhiza isolate
摘要
Abstract
Salvia miltiorrhiza is one of the major traditional Chinese medicinal plants in China.Virus diseases are widespread in S.miltiorrhiza in Henan province.Soybean geminivirus B(SGVB)was first detected in S.miltiorrhiza in Henan.To elucidate the genomic structure and genetic diversity of the soybean geminivirus B Salvia miltiorrhiza isolate(SGVB-SM),the complete genome sequences of eight SGVB-SM isolates were obtained using PCR.The genome of SGVB-SM is 2 625 nucleotides(nt)in length and encodes four proteins:a 256-amino acid(aa)coat protein(CP),a 97-aa movement protein(MP),a 163-aa C1 protein,and a 293-aa C2 protein.Nucleotide sequence identity analysis revealed that the eight SGVB-SM isolates shared 93.5％to 95.0％identity across the whole genome with the Korean SGVB-Kong isolate.The nucleotide identities of CP,MP,C1,and C2 ranged from 93.9％to 98.3％,88.4％to 95.2％,93.3％to 95.9％,and 93.7％to 95.6％,respectively,while the corresponding amino acid identities were 93.1％to 100％,90.5％to 100％,91.7％to 100％,and 100％,respectively.Further analysis of CP sequences from 33 SGVB-SM isolates demonstrated that nucleotide and amino acid identities among these isolates were 90.9％to 96.5％and 92.2％to 94.9％,respectively.Compared with the Kong isolate,the nucleotide and amino acid identities ranged from 93.9％to 98.3％and 93.1％to 100％,respectively.The CP gene of SGVB-SM isolates is 768 nt in length.Phylogenetic analysis showed that SGVB-SM isolates clustered in the same branch as the SGVB-Kong isolate.Recombination analysis revealed seven recombination events in six SGVB-SM isolates,with recombination regions mainly located between 1 040 nt and 2 618 nt.This study enriches the sequence information of SGVB and contributes to a better understanding of the genetic evolutionary relationships of this virus population.关键词
丹参/大豆双生病毒B/基因组结构/遗传变异/重组分析Key words
Salvia miltiorrhiza/soybean geminivirus B/genomic structure/genetic variation/recombinant analysis分类
农业科技引用本文复制引用
徐国豪,秦艳红,刘红彦,鲁书豪,李想,高素霞,文艺,李绍建,杨瑾,李雪梦,王飞..大豆双生病毒B丹参分离物的基因组结构分析及遗传变异研究[J].植物保护,2026,52(3):143-151,9.基金项目
国家中药材产业技术体系(CARS-21) （CARS-21）
河南省中药材产业技术体系(HARS-22-11-Z1) （HARS-22-11-Z1）
河南省科技攻关项目(252102111114) （252102111114）
河南省农业科学院新兴学科项目(2024XK06) （2024XK06）
